摘要
目的 探讨米托蒽醌治疗白血病的机制。方法 应用细胞培养，细胞形态学观察，ＤＮＡ琼脂糖凝胶电泳等技术进行检测和观察。结果 ＨＬ－６０白血病细胞在米托蒽醌的作用下，出现典型的凋亡特征，细胞形态学表现出细胞核裂解，染色质聚集，核碎裂，胞浆浓缩，有空泡形成，ＤＮＡ琼脂糖电泳出现典型梯形条纹。结论 米托蒽醌通过诱导白血病细胞凋亡起到治疗白血病的作用。
To investigate the mechanisms of mitoxantrone killing leukemia cells. Methods Culture HL 60 cells, cell morphollogy, DNA agarose gel electrophoresise were performed. Results HL 60 leukemia cells treated with mitoxantrone underwent apoptosis. Apoptosis cells demonstrated compaction and segregation of the nuclear chromatin, condensation and vacuolus of the cytoplasm. Nuclear DNA of apoptosis cells display ladder bands charateristic of internucleosomal DNA fragmentation. Conclusion The mechanism of mitoxantrone killing leukemia cells is inducing apoptos is. Key Words mitoxantrone; leukemia; apoptosisis medicines,abuse of antibiotics and incorrect conception of consumption.It is suggested that the effective measures to control the over-consuming of medicines in our country are to adhere to the policy of the development of non-patent medicines and the practice of unified purchase and marketing of medicines,cut off unfair competition,and illegal way of selling medicines,work out a name list of basic medicines,attach great importance to economical evaluation,carry out standardized medical care,control hospital infection,change or correct the conception of consumption. Key words:consumption of medicines;cause;corresponding policynce and Technology Department of Tsinghua University,Pejing 100084) Abstract DNA based immunization against hepatitis B surface antigen was used as a model system for the studies of the immune responses Mice were immunized with genes encoding the hepatitis B surface antigens by intramuscular,intraperitoneal,and epidermal scratch routes The changes of anti HBsAg antibodies in the sera of mice were tested by ELISA It was found that the plasmid DNA induced the highest level of antibody responses by epidermal scrach route over those of intramuscular and intraperitoneal inoculations in mice
出处
《实用儿科临床杂志》
CAS
CSCD
1999年第5期281-281,295,共2页
Journal of Applied Clinical Pediatrics
关键词
米托蒽醌
白血病
药物疗法
gene immunization
hepatitis B surgace antigen
inoculation route
