摘要
硝基呋喃类药物的检测常以其代谢物为目标物,建立了鸡肉、蜂蜜、牛奶中呋喃唑酮代谢物(AOZ)、呋喃它酮代谢物(AMOZ)、呋喃妥因代谢物(AHD)和呋喃西林代谢物(SEM)检测的超高效液相色谱串联质谱确证方法,硝基呋喃代谢物经衍生化后,采用硅藻土固相支撑液液萃取和平行蒸发联用前处理技术进行净化和富集衍生物,超高效1.7μm C18柱用于分离4种待测物,同位素内标法定量。AOZ和AMOZ为最低检测限为0.1μg/kg,AHD和SEM为0.25μg/kg。3个不同添加水平时该方法回收率为85.6%～104.3%,相对标准偏差(RSD)为3.2%～9.5%,通过对实际样品进行测定,结果表明该方法能简单、快速、准确地测定动物源性食品中4种硝基呋喃类代谢物。
A confirmative method was developed to determine nitrofuran metabolites,which is usually selcted as analystes,in animal-origin foodsutffs by UPLC-MS/MS.The metabolites was derivated and purified with diatomite solid-supported-liquid-liquid extraction（SSLLE） and parallel evaporation（PE）.The separation is carried on 1.7 μm C18 column and quantified with isotope internal standard.The limit of quantication（LOQ） was 0.1 μg/kg for AOZ and AMOZ,0.25 μg/kg for AHD and SEM,respectively.The avearage recoveries ranged from 85.6% to 104.3% spiked wih three level,and the RSD from 3.2% to 9.5%.Apllied to real sample,it proved that the method was simple,fast,and accurate for determining 4 nitrifurans metabolites in aninal-origin foodstuffs.
出处
《食品科技》
CAS
北大核心
2011年第2期300-303,共4页
Food Science and Technology
基金
河南省普通科技攻关项目(0524430004)
