摘要
目的探讨骨形态发生蛋白-4(BMP-4)单核苷酸多态性与颈椎后纵韧带骨化(OPLL)的遗传关联性。方法 OPLL病例组和正常对照组各200例,利用聚合酶链反应(PCR)和直接测序法分析BMP-4上4个单核苷酸多态性位点6007C>T(rs17563),-5826G>A(rs1957860),3564C>T(rs2855532)和IVS-160C>T(rs2071047)基因型及等位基因的分布。结果 两组间位点rs17563、rs2855532基因型与等位基因型有显著性差异(P<0.05)。病例组中rs17563带C+基因型与C-基因型骨化椎体数量有非常高度显著性差异(P<0.001),前者骨化椎体数量明显少于后者;rs2855532T+基因型与T-基因型骨化椎体数量有显著性差异(P<0.05),前者骨化椎体数量多于后者。余两位点无显著性差异(P>0.05)。结论 BMP-4基因的两个单核酸多态性位点rs17563、rs2855532等位基因型突变与OPLL的发生和发展有关。
Objective To explore the relationship of single nucleotide polymorphism（SNPs） of the bone morphogenetic protein-4（BMP-4） gene with the morbidity and severity of the ossification of posterior longitudinal ligament（OPLL） of the spine in Chinese patients.Methods The venous blood samples of 200 OPLL patients and 200 non-OPLL controls were measured with the DNA sequences in four locus of BMP-4 gene with polymerase chain reaction（PCR）.Results There was a significant association between the rs17563,rs2855532 polymorphism and the occurrence of OPLL（P0.05）.The ＇＇T＇＇ allele in these 2 SNPs promotes the extent of OPLL（P0.05）.However,no significant association in morbidity and severity was found between the other 2 SNPs（rs1957860,rs2071047）.Conclusion SNPs in rs17563,rs2855532 associates with the morbidity and severity of the ossification of posterior longitudinal ligament of the spine in Chinese patients.
出处
《中国康复理论与实践》
CSCD
2011年第1期39-41,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金(30872599)
北京市自然科学基金(7092028)
关键词
后纵韧带骨化症
骨形态发生蛋白-4
基因
单核苷酸多态性
ossification of posterior longitudinal ligament
bone morphogenetic protein-4
gene
single nucleotide polymorphism
