摘要
目的:探讨地知复方治疗糖尿病(DM)的主要有效部位及作用机制。方法:一次性腹腔注射链脲佐菌素(STZ)造成糖尿病小鼠模型。采用水提醇沉法将该方分为20%醇溶部位(20%CR),80%醇沉部位(80%CC)和80%醇溶部位(80%CR)。测定给药前后1小时血糖;血清、肾组织的NO和丙二醛(MDA)含量。结果:灌胃给药1小时后,各给药组与DM组相比血糖变化差异均有非常显著性(P<0.01)。与DM组相比,各治疗组血清NO水平均显著下降。肾组织中NO水平,各治疗组均有下降。血清、肾组织MDA含量各给药组均较DM组有非常显著下降。结论:80%CC和80%CR两个部位对于氧化损伤指标的改善基本上接近原方的作用。从血清、肾MDA,血清NO和体质量变化指标来看,80%CC部位和80%CR部位的药效均不及20%CR部位,提示这两个部位对于上述药效指标似乎有协同作用。但是80%CC部位的降血糖作用远远强于80%CR,也强于20%CR部位,提示该复方的降血糖作用主要来源于80%CC部位。
Objective：To study the main effective parts and mechanisms of Dizhi formula for treating diabetes mellitus（DM）.Methods：The experimental diabetic model was established by intraperitoneal injection of STZ（170mg/kg） in mice after fasted for 13 h.Dizhi formula was divided into 3 parts by water extracting and alcohol precipitation.They were the fraction from aqua extract precipitated by 20% ethanol filtered and concentrated（20%CR）,the fraction from aqua extract precipitated by 80% ethanol（80%CC）,the fraction from aqua extract precipitated by 80% ethanol filtered and concentrated（80%CR）.The blood glucose（before and after administration）were assayed.The contents of NO,MDA in serum and kidney were assayed.Results：At 1h after administration,the blood glucose decreased in DM-20%CR,DM-80%CC and DM-80%CR groups compared with DM group.Compared with DM group,all the drug groups decreased the content of NO,MDA in serum and kidney tissues.Conclusion：The activities of inhibiting oxidative stress of 80%CC and 80%CR parts were close to Dizhi formula.However,the activity of 20%CR was stronger.It suggests that 80%CC and 80%CR parts have synergistic action in improving oxidative stress.80%CC part has the far more powerful hypoglycemic effect than 80%CR part,even than 20%CR part.It could be supposed that the hypoglycemic activity mostly comes from 80%CC part.
出处
《现代医药卫生》
2011年第2期163-165,共3页
Journal of Modern Medicine & Health
