摘要
目的建立薄荷饮片挥发油的气相色谱(GC)指纹图谱。方法采用HP-INNOWax毛细管柱(30 m×0.32 mm,0.25μm),进样口温度250℃,检测器温度为280℃,炉温采用程序升温,初始温度100℃,保持15 min后,以8℃/min升至140℃后保持10 min,再以5℃/min升至200℃,载气流速为1.3 mL/min,分流比为2:1。结果 10批薄荷饮片挥发油标示出13个共有峰,5号峰(薄荷脑)的峰面积百分含量为(65.78±13.90)%。结论该方法准确可靠、重复性好,为薄荷饮片质量控制提供了一定的参考。
Objective To establish the method of fingerprint analysis on volatile oil of the decoction pieces of Mentha Haplocalyx Herba by GC.Methods The HP-INNOWax capillary column（30 mm×0.32 mm,0.25 μm） was used with the sampling temperature at 250 ℃ and the FID detector temperature at 280 ℃.The temperature programming was adopted with the initial column temperature at 100 ℃,which was kept for 15 min,then increased by 8 ℃/min^-1 to 140 ℃,after keeping for 10 min,reincreased by 5 ℃/min^-1 to 200 ℃.The flow rate of the carrier gas was 1.3 mL/min^-1 with the split ratio of 2 ∶1.Results 13 common peaks were separated on GC fingerprint spectrum in 10 batches of the decoction pieces of Mentha Haplocalyx Herba.The percentage composition of peak area in peak 5（menthol） was（65.78±13.90）%.Conclusion The method is reliable and accurate with good reproducibility,which provides certain reference for controlling the quality of Mentha Hapiocalycx Herba.
出处
《中国药业》
CAS
2011年第4期28-30,共3页
China Pharmaceuticals
基金
"十一五"国家科技支撑计划项目
项目编号:2008BAI53B074
关键词
薄荷
挥发油
指纹图谱
气相色谱法
Mentha Haplocalyx Herba
volatile oil
GC
fingerprint spectrum
