摘要
采用垂直板式聚丙烯酰胺凝胶电泳技术对分别来自中国青岛与日本北海道海带（Ｌａｍｉｎａｒｉａｊａｐｏｎｉｃａ）丝状体的６种酶系统（乙醇脱氢酶、谷氨酸脱氢酶、异柠檬酸脱氢酶、乳酸脱氢酶、山梨醇脱氢酶和超氧化物歧化酶）进行了检测，发现存在２２个基因位点，分析了不同来源海带丝状体的遗传多样性与遗传分化程度。结果表明：日本北海道海带丝状体的预期杂合度、每个位点有效等位基因数等遗传参数均大于中国青岛海带丝状体。对日本北海道海带丝状体与中国青岛海带丝状体的遗传相似系数和遗传距离进行了计算分析，显示两者发生了遗传分化。日本北海道海带丝状体的遗传多样性高于中国青岛海带丝状体。研究结果还发现在中国青岛海带丝状体的Ｉｄｈ、Ｌｄｈ和Ｓｄｈ中，以及日本北海道海带丝状体的Ａｄｈ、Ｇｄｈ、Ｉｄｈ、Ｌｄｈ、Ｓｄｈ和Ｓｏｄ中存在着不同迁移率的不等距双带酶，可能由特殊基因控制，并集中在第３个基因位点上。
Genetic diversity was
compared and analyzed between Laminaria japonica gametophytes from Qingdao of China
and those from Hokkaido of Japan. Six enzyme systems (Adh,Gdh, Idh, Ldh, Sdh, and Sod) were
examined by PAGE, and twenty two gene loci were found. The results showed that the genetic
diversity in the gametophytes of Hokkaido was higher than that of Qingdao. There was genetic
differentiation in gametophytes between Hokkaido and Qingdao. Double banding patterns of
allozymes with different mobility were found for Qingdao gametophytes in Idh, Ldh, and Sdh
enzymes as well as for Hokkaido gametophytes in all six enzymes. The bands may be
controlled by special genes which mainly exist in the third gene loci.
出处
《生物多样性》
CAS
CSCD
1999年第3期197-201,共5页
Biodiversity Science
基金
国家自然科学基金
