摘要
用焦锑酸钾沉淀法进行了组织和细胞中游离钙的化学定位。用光学显微镜和透射电镜观察石刁柏幼苗在太空飞行后Ｃａ２＋沉淀颗粒在根尖组织和细胞内的分布。结果表明，太空飞行１５天后，Ｃａ２＋在各组织内的分布情况与地面对照无明显差异，但Ｃａ２＋的含量明显低于对照。Ｃａ２＋在细胞内不同区域的分布在飞行和对照样品中差异十分明显，对照细胞中Ｃａ２＋集中在液泡内，其它细胞器中很少见到。飞行幼苗的根尖细胞，液泡中Ｃａ２＋很少，并向液泡膜集结，液泡膜内侧和细胞质中的Ｃａ２＋明显增多。细胞壁中的Ｃａ２＋较对照有明显增加，高尔基体中也有少量钙存在。本文着重讨论了飞行幼苗根尖中Ｃａ２＋在细胞内重新分布的可能作用。
The distribution of Ca2+ sediments in root tip tissues and cells of Asparagus officinalis seedlings after 15-days spaceflight was studied by light and electron microscopy. It is shown that the Ca2+ distribution in root tip tissues of the spaceflight samples had no distinct difference from the ground control,but Ca2+ concentration in them was remarkabley lower than the control. The Ca2+ distribution within cell was notably different. In the control cells, Ca2+ concentrated to vacuole and was less in other cellular organelles. But, in the spaceflight samples, Ca2+ was concentrated to the vacuole membrane with a linear pattern, less Ca2+ was in the vacuoles. Ca2+ increased remarkably in cytoplasm and inner lateral of vacuole membrane. Ca2+ in cell walls notably increased than in control. Some Ca2+ was found in the Golgi complex. This article emphatically discusses the possible mechanism of Ca2+ redistribution in the root tip cells of spaceflight seedlings .
出处
《生物物理学报》
CAS
CSCD
北大核心
1999年第2期381-386,共6页
Acta Biophysica Sinica
