摘要
以２－溴－１，１－二乙氧基乙烷为起始物，制备了牛磺酸的一种类似物乙醛磺酸（ｓｕｌｆｏｎｉｃａｌｄｅｈｙｄｅ；ＳＡＤ），并对其部分化学性质、光吸收以及细胞半致死量进行了测定。所合成的ＳＡＤ在２０３ｎｍ和２５０ｎｍ分别有吸收，在ＨＰＬＣ分离谱上为单一的峰。ＳＡＤ在碱性溶液中不稳定，在酸性条件下相对稳定。ＳＡＤ对中国仓鼠卵巢细胞（ＣＨＯ）的生长具有抑制和毒性作用，其半致死量为３００μｍｏｌ／Ｌ。由于ＳＡＤ含有较为活泼的醛基，可与肼及氨基反应，因此，ＳＡＤ的合成为我们制备新的牛磺酸类化合物打下了基础。
Sulfonic aldehyde(SAD), an analogue of taurine, was synthesized from 2-bromo-1, 1-diethoxyethane(BDE) and characterized. SAD showed a single peak by HPLC analysis with a C-18 column. The maximum absorbance of SAD was at 203nm and 250nm. Because of the active aldehyde group, SAD reacted with the group of -NH-NH2 and -NH2, and produced DNP-EHSA and Schiff's base derivatives. SAD resulted in a new absorbance at 230nm with dwindling of its absorbance at both 203nm and 250nm when it was incubated in phosphate buffer(pH 7.3) at room temperature. However, no change in the absorbance peak could be detected while SAD was incubated in the same buffer at pH5. It was observed that SAD was cytotoxic to CHO cells and the median lethal dose of SAD to the cells was 300μmol/L. Since taurine demonstrated important physiological functions and was an effective medicine, the results reported here were instrumental in further studying functions of taurine and screening of therapeutic analogues of taurine.
出处
《生物物理学报》
CAS
CSCD
北大核心
1999年第2期320-326,共7页
Acta Biophysica Sinica
关键词
牛磺酸
乙醛磺酸
卵巢细胞
制备
生长
Sulfonic aldehyde Chinese hamster ovary cell cell growth nerves
