摘要
本文报道乙醛磺酸（ＳＡＤ）不仅会降低小鼠胚胎细胞的ｒＲＮＡ基因转录活性，而且还明显提高了胚胎细胞姐妹染色单体互换（ＳＣＥ）频率，导致胚胎发育停滞和胎儿死亡，最终使幼鼠出生率明显下降。当ＳＡＤ浓度为１０－５ｍｏｌ／Ｌ时，小鼠胚胎细胞ｒＲＮＡ基因转录活性降低为正常值的１５％，并引起ＳＣＥ频率成倍增加，幼鼠出生率仅为正常值的５０％；当ＳＡＤ浓度增至１０－３ｍｏｌ／Ｌ时，此时ｒＲＮＡ基因转录活性完全被抑制，而且ＳＣＥ频率增加为正常值的４．８倍，幼鼠出生率从正常值１００％降为４％。ＳＡＤ对小鼠胚胎细胞这种细胞遗传学效应和对胚胎发育影响的程度与该药浓度成正相关。此外，我们还对ｒＲＮＡ基因转录活性和ＳＣＥ的改变与哺乳类胚胎发育之间的关系进行了初步的讨论。
We report here that SAD may not only suppress transcription activity of rRNA genes, but also increase SCE frequency of mouse embryonic cells, leading to bloching of the embryo development and fetal death, and finally significant decrease of birth percentage of the new born offsprings Ata concentration of SAD in 1 0-3mol / L, the transcription activity of rRNA genes was decreased to 1 5% of the normal level, and the SCE frequency was increased doubly, leading to that the birth percentage was decreased to 50% of normal one. When SAD concentration 8f 1 0-3 mol / L was used the transcription activity of rRNA genes was completely suppresed and SCE frequency was increased to as 4.8 times high as normal, leading to that the birth percentage dropped to 4% from 100% . Our results indicates that both the cytogenetic effects and the inhibition of the embryonic cells are directly proportional to the SAD concentration used. The relationship between the change of both the rRNA gene transcription and SCE frequency and the degree of embryo development are also disccusesed preliminarily.
关键词
乙醛磺酸
胚胎
发育
细胞遗传
鼠
Sulfonic aldehyde, Sister chromatid exehange, NOR activity, Embryo development
