摘要
建立了同时测定香兰素和邻位香兰素的高效液相色谱法。考察了流动相组成、柱温等因素对分析效率的影响。在流动相为5％乙酸-乙腈（60：40，体积比），流速1．0mL／min，柱温25℃的优化条件下，香兰素和邻位香兰素可在5min内实现分离。测定结果表明，香兰素和邻位香兰素在10～240mg／L范围内线性关系良好，相关系数均为0．9997；方法的检出限（S／N=3）分别为0．06、0．02mg／L；样品的加标回收率为99％～102％，相对标准偏差为0．2％～0．6％。该方法操作简单、快速，用于实际样品的分析，结果满意。
A high performance liquid chromatographic method was developed for the simultaneous separation and determination of vanillin and o-vanillin. Influences of mobile phase composition and column temperature on the separation were investigated. Under the condition of 5% acetic acid - acetonitrile （60 ： 40, by volume） as mobile phase at a flow rate of 1.0 mL/min and 25 ℃, the vanillin and o-vanillin were separated in less than 5 min. The calibration curves of vanillin and o-vanillin showed good linearities in the range of 10 - 240 mg/L with both correlation coefficients of 0. 999 7. The limits of detection（ S/N = 3 ） for vanillin and o-vanillin were 0.06 mg/L and 0.02 mg/L, respectively. The average recoveries at three spiked levels were in the range of 99% -102% with relative standard deviations of 0. 2% - 0.6%. The method was successfully used for the determination of vanillin and o-vanillin in real samples with satisfactory results.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第2期222-224,228,共4页
Journal of Instrumental Analysis
基金
辽宁省自然基金资助项目(20090655)
辽宁省教育厅2008年度科研计划项目(2008S184)
