摘要
目的:探讨眼针疗法治疗缺血性脑血管病的可能机制。方法:采用随机分组将大鼠分为假手术组、模型组与眼针组,改良的线栓方法制作大鼠局灶性脑缺血再灌注模型;取眼针上焦区、下焦区、肝区、肾区为施加因素;采用神经功能缺损评分,TTC染色测量梗死体积;电镜下观察缺血半暗区脑组织超微结构;结果:眼针组与模型组比较神经功能缺损评分、梗死体积均有显著差异(P<0.01);电镜下观察到眼针组缺血半暗区脑组织神经细胞元、神经胶质细胞、毛细血管内皮等结构均较模型组损伤轻。结论:眼针可降低脑缺血再灌注损伤大鼠神经功能缺损评分,缩小脑梗死体积,改善缺血半暗带区脑组织超微结构损伤,从而对脑缺血再灌注起保护作用。
Objective：To probe the possible mechanism of eye-acupuncture in the treatment of Ischemic Cerebrovascular Disease.Methods：Divided the rats into 3 groups by random,that is,the sham-operated group,model group and eye-acupuncture group,Make the model rat of focal cerebral ischemia-reperfusion according to the improved method;to take on the Shangjiao area,Xiajiao area,liver area,kidney area for the imposition factors;Make the scores of neurological function deficits,adopt the TTC staining to measure the infarct volume;observe the ultra-structure of the brain tissue in ischemic penumbra by electron microscope.Results：eye-acupuncture group compared with model group are significantly different（P〈0.01）at the scores of neurological function deficits and the infarct volume;electron microscope observation neuronal of the brain tissue in ischemic penumbra,glial cells,capillary endothelial etc structure all light damage than those in model group.Conclusion：Eye-acupuncture can reduce the scores of neurological function deficit of the cerebral ischemia-reperfusion rats,decrease the infarct volume,improve the injury of ultra-structure of the brain tissue in penumbra and at the same time protect the impaired brain caused by ischemia-reperfusion.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第2期360-363,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(2007CB512702)
辽宁省科技厅自然基金项目(20052065)
关键词
缺血再灌注
梗死体积
神经功能缺损评分
眼针疗法
超微结构
ischemia- reperfusion
infarct volume
scores of neurological function deficit
the therapy of eye- acupuncture
