摘要
在pH8.6-9.9的条件下，苯唑西林（OXA）与甲基紫（MV）发生反应生成紫色离子缔合物，产生明显的退色现象，其最大和次大负吸收波长分别位于529和606nm，线性范围均为0-4．1μg／mL，表观摩尔吸光系数（ε）分别为2．63×10^4L·mo^-l ·cm^1（λ=529nm）和2．28×10^4L·mol^-1·cm^-1（λ=606nm），苯唑西林在一定浓度范围内服从比尔定律，据此建立了测定苯唑西林的退色分光光度法。用于市售苯唑西林药物中苯唑西林的测定，结果满意。
At a pH value of 8.6～9.9,oxacillin(OXA) can react upon methyl violet(MV) to form purple ion association complexes and cause obvious fading.The maximum negative absorption wavelength and sub-major negative absorption wavelength of the system were 529 nm and 606 nm,respectively,and their linear range was 0～4.1 μg/mL and apparent molar absorptivity(ε) was 2.63×104 L·mol-1·cm-1(529 nm)and 2.28×104 L·mol-1·cm-1(606 nm),respectively.Oxacillin obeys the Beer′s law in a definite concentration range.A novel washed-out spectrophotometric method for the determination of oxacillin was thereby developed.This method has been applied to the determination of oxacillin content in the commercially available oxacillin medicament with satisfactory results.
出处
《化学试剂》
CAS
CSCD
北大核心
2011年第2期142-144,共3页
Chemical Reagents
基金
重庆市教委科技基金资助项目(KJ101315)
长江师范学院科研资助项目(2009xky008)
关键词
苯唑西林
甲基紫
退色反应
分光光度法
oxacillin
methyl violet
color fading reaction
spectrophotometry
