摘要
目的研究齐墩果酸（oleanolic acid,OA）诱导人结肠癌LoVo细胞株凋亡的机制。方法应用浓度为2.00~32.00 mg.L-1OA作用于人结肠癌LoVo细胞12~96 h后,采用噻唑蓝（MTT）法检测OA对LoVo细胞的抑制作用,采用流式细胞术、Western blot印迹法等检测细胞周期变化、凋亡及Bcl-2、Bax、p53、Caspase-3蛋白表达,在光镜及电镜下观察细胞形态学变化。结果 OA呈时间-剂量依赖性抑制LoVo细胞增殖,能诱导LoVo细胞凋亡及阻滞细胞于G2/M期,OA作用LoVo细胞48 h后,各组的细胞凋亡率为10.32%~14.24%（P〈0.05）,并出现Bcl-2蛋白表达抑制,p53、Bax及Caspase-3活性增强。结论 OA可以促进人结肠癌LoVo细胞凋亡,其促凋亡机制与抑制Bcl-2表达及促进p53、Bax及Caspase-3表达有关。
Objective To study the effects of oleanolic acid（OA） on proliferation and apoptosis of colon cancer LoVo cell lines in vitro. Methods The human colon cancer LoVo cells were treated with 2.00～32.00 mg·L-1 OA for 12～96 h.The influence of OA on proliferation of LoVo cell in vitro was detected with MTT method.Cell apoptosis rate was determined by flow cytometry,and protein expressions of Bcl-2,Bax,p53 and Caspase-3 were investingated by Western blot analysis.The apoptosis was observed by electron microscopy. Results OA inhibited the proliferation of LoVo cells in a dose and time dependent way.It significantly induced apoptosis of LoVo cells and blocked cells at G2/M phase.The apoptosis rates were 10.32%～14.24%（P0.05） after 48 hours.With the increase of concentrateion of OA,the expressions of Bax,p53 and Caspase-3 were increased,while the level Bcl-2 protein was decreased. Conclusion OA induces apoptosis of colon cancer LoVo cell lines,which may be related to inhibition of Bcl-2 and promotion overexpressing of Bax,p53 and Caspase-3.
出处
《医药导报》
CAS
2011年第2期176-180,共5页
Herald of Medicine
