摘要
以废水处理生物膜为研究对象,建立了表征生物膜细胞活性的磷脂测定方法.研究中采用冷冻切片技术,以150μm为一层,考察生物膜不同深度的磷脂浓度.结果表明,当五氯酚浓度为零时,生物膜磷脂含量随深度方向呈近似的正态分布,磷脂含量的绝对值在(1246.2±217.4)～(527.2±95.3)μg/L之间(n=4),峰值出现在生物膜深度(0.60±0.15)μm位置,表明该处生物膜活细胞含量最高.当五氯酚浓度分别为5、10和20μg/L时,控制暴露时间为2、6和24 h后,结果表明,除5μg/L五氯酚暴露2 h的情况之外,五氯酚作用下的生物膜各层磷脂分布值明显下降,尤其在0.7～0.9μm附近,磷脂的降低程度最大可达70%.实验数据表明五氯酚对生物膜内细胞存活情况具有不利影响,且随着PCP浓度及暴露时间的增加,不利影响的程度增强.
The phospholipids method for the determination of the living cell content in the biofilm was established to study the wastewater biofilm.With the micro-slicing technology,the biofilm was divided into 150μm-layer for the phospholipids analysis.Results showed the phospholipids were approximately normal distribution along the biofilm depth without no addition of pentachlorophenol(PCP).The absolute values were between(1 246.2 ± 217.4) μg/L and(527.2 ± 95.3) μg/L with the maximum occurring at the depth of(0.60 ± 0.15) μm,indicating the highest concentrations of the living cell in the biofilm.With the PCP concentrations of 5 μg/L,10 μg/L and 20 μg/L and the exposure times of 2 h,6 h and 24 h,respectively,the results of the orthogonal experiments showed the value of phospholipids decreased greatly along the biofilm depth only with the exception of 5 μg/L PCP concentration and 2 h exposure time,with a concentration decline by up to 70% especially close to the peak of phospholipids distribution.Results proved that PCP has an adversely influence on the living cells,and the influence can be enhanced with the increase of PCP amount and the exposure time.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2011年第2期483-487,共5页
Environmental Science
基金
浙江省自然科学基金项目(Y5080009)
关键词
磷脂测定
五氯酚
微切片
生物膜
活细胞含量
phospholipids method
pentachlorophenol(PCP)
micro-slicing
biofilm
living cell content
