摘要
目的:探讨黄芪-脉络宁胶原对血管新生的细胞学影响及其可能机制,为其应用于临床提供理论依据。方法:应用增殖、迁移、管腔形成实验观察黄芪-脉络宁胶原对大鼠胸主动脉内皮细胞在血管新生各阶段的作用,用Western Bloting实验检测大鼠胸主动脉血管内皮细胞生长因子(VEGF)的表达率。结果:黄芪-脉络宁胶原能明显促进大鼠胸主动脉内皮细胞的增殖率(与对照组比较,增加了90.22%,P<0.01)、迁移率(与对照组比较增加了211.43%,P<0.01)、管腔形成数(是对照组的2.46倍,P<0.01),同时刺激VEGF蛋白表达比对照组增加63.8%(P<0.01)。结论:黄芪-脉络宁胶原能明显促进内皮细胞增殖、迁移、管腔形成,具有显著促体外血管新生作用,其机制可能与增加VEGF蛋白的表达有关。
Objective:To explore the angiogenic effects and possible mechanisms of modified collagen matrices loading with Huangqi-Maluoning injection with cell experiments.Method:2 groups of collagen matrices loading with or without Huangqi-Mailuoning injection were set in the experiments,each of which was cultured with thoracic aorta endothelial cells for 24 hours. Afterwards,the effects of cell proliferation,migration and tube formation were detected followed by protein expression of VEGF with western blot assay.Results:The group of collagen matrices loading with Huangqi-Mailuoning injection can positively increase rat thoracic aorta endothelial cell proliferation to 1.9022 times,migration to 2.1143 times and tube formation to 2.46 times as compared to the results of group of collagen without loading Huangqi-Mailuoning injection. The protein expression of VEGF improved 0.638 time in the group of collagen matrices loading with Huangqi-Mailuoning injection as compared to that without loading.Conclusion:The angiogenic effects of collagen matrices loading with Huangqi-Mailuoning injection are by way of stimulating proliferation,migration and tube formation of thoracic aorta endothelial cells. One of the possible mechanism may through upgrade the protein expression of VEGF.
出处
《微循环学杂志》
2011年第1期8-11,F0003,I0001,共6页
Chinese Journal of Microcirculation
基金
江苏省自然科学基金(BK2007175)
江苏省"六大人才高峰"(06-B-071)
