摘要
目的:探讨电针预处理对肝脏缺血再灌注损伤后的作用及作用机制。方法:24只国产大耳白兔,随机分为假手术(S)组、缺血再灌注(IR)组、电针预处理(EAP+IR)组,每组8只。EAP+IR组、IR组建立肝缺血再灌注损伤模型,EAP+IR组于术前电针刺激"阳陵泉"穴30 min。在缺血前及IR后8 h、24 h处死动物,取右肝叶进行一氧化氮(NO)及诱导型一氧化氮合酶(iNOS)测定,从耳缘动脉抽血2 mL行丙二醛(MDA)及超氧化物歧化酶(SOD)检测。结果:再灌注8 h、24 h后,EAP+IR组的NO为(0.491±0.102)μmol/g、(0.891±0.102)μmol/g,MDA为(5.94±0.35)μmol/L、(6.81±0.25)μmol/L,iNOS为(0.361±0.251)U/mg、(1.173±0.331)U/mg,活力较IR组明显下降[(0.756±0.087)μmol/g、(1.171±0.098)μmol/g、(6.36±0.21)μmol/L、(9.65±0.34)μmol/L、(0.692±0.223)U/mg、(1.711±0.323)U/mg](P<0.05,P<0.01),SOD活力增加(P<0.01);与S组比较,EAP+IR及IR组NO、MDA含量及iNOS活力增加,SOD活力下降(均P<0.01)。结论:电针预处理可以抑制肝组织中iNOS的产生,降低NO、MDA含量,提高SOD活力,从而保护肝组织。
Objective To explore the effect of electroacupuncture preconditioning on hepatic ischemia-reperfusion injury and its mechanism. Methods Twenty-four rabbits were randomly divided into a sham operation （S） group, an ischemia-reperfusion （IR） group and an electroacupuncture preconditioning plus isehemia-reperfusion （EAP＋ IR） group, 8 cases in each group. Models of hepatic ischemia-reperfusion injury were produced in the groups EAP＋ IR and IR, and the rabbits in the group EAP＋IR were treated with preoperative eleetroacupuncture at ＂Yanglingquan＂ （GB 34） for 30 rain. The rabbits were sacrificed before ischemia or at 8 h, 24 h after ischemia-reperfusion, and the right hepatic lobes were collected to detect the content of nitric oxide （NO） and the induced nitric oxide synthase （iNOS）. Moreover, blood samples （2 mL） were drawn from ear marginal artery to detect malondialdehyde （MDA） and superoxide dismutase （SOD）. Results At 8 h and 24 h after hepatic ischemia-reperfusion, the content of NO[（0.491 ± 0.102） umol/g, （0.891 ± 0.102） umol/g], MDA[（5.94 ± 0.35） umol/L, （6.81± 0.25）umol/L）] and the activity of iNOSE（0. 361 ± 0. 251 ） U/mg, （ 1. 173 ± 0. 331 ） U/mg] in the group EAP＋ IR were significantly lower than those in the group IR [（0. 756 ± 0. 087） umol/g, （ 1. 171 ± 0. 098） umol/g, （6.36 ± 0.21）umol/L,（9.65 ±0.34）umol/L,（0.692±0.223）U/mg,（1.711±0.323）U/mg,P〈0.05, P〈0.01], but the activity of SOD was significantly increased（P〈0.01）. Compared with group S, the content of NO, MDA and the activity of iNOS in the groups EAP＋ IR and IR were significantly increased, but the activity of SOD were significantly decreased （all P〈0.01）. Conclusion Electroacupuncture preconditioning can inhibit the production of iNOS in liver, reduce the content of NO and MDA, and increase the activity of SOD so as to protect the liver.
出处
《中国针灸》
CAS
CSCD
北大核心
2011年第2期149-152,共4页
Chinese Acupuncture & Moxibustion
