摘要
取成年SD大鼠的下丘脑、垂体、卵巢组织制成石蜡切片,采用PV-9000两步法免疫组织化学检测GHS-R1a在下丘脑-垂体-卵巢轴上的定位。实时荧光定量PCR检测GHS-R1a mRNA在不同发情周期大鼠下丘脑-垂体-卵巢轴上的表达。结果表明:GHS-R1a主要分布在下丘脑的弓状核、腹内侧核、正中隆起等,腺垂体的嗜色细胞,卵巢的黄体细胞内、卵母细胞、卵巢门间质细胞内。GHS-R1a mRNA在不同发情周期大鼠的下丘脑、垂体中均有表达,且受发情周期的控制,在整个发情周期大鼠的卵巢中均未检测到GHS-R1a mRNA的表达。
The distribution of GHS-R1a in hypothalamus-pituitary-ovarian axis were detected by PV-9000 two-step immunohistochemical.Real-time quantitative PCR was used to detect expression of GHS-R1a mRNA during estrous cycle.The results showed that GHS-R1a were mainly distributed in hypothalamus of the arcuate nucleus,ventromedial nucleus,median eminence,the chromophil cells of anterior pituitary,luteal cells,hilus interstitial cells,and oocyte.GHS-R1a mRNA were expressed in hypothalamus and pituitary of rats throughout the estrous cycle and regulated by estrous cycle.GHS-R1a mRNA was not observed in ovary of rats.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第1期39-43,共5页
Journal of Huazhong Agricultural University
基金
安徽省自然科学基金项目(070411015)
