摘要
向人血浆中加入１μｍｏｌ／Ｌ亚甲蓝，以１００００ｌｘ强度的溴钨灯光照射５ｍｉｎ，可灭活１０６５ＴＣＩＤ５０的水泡性口炎病毒与１０７４１ＴＣＩＤ５０的辛德毕斯病毒；作用２ｍｉｎ可灭活１０５０９ＴＣＩＤ５０的人免疫缺陷１型病毒。但作用６０ｍｉｎ仍不能破坏ＨＢｓＡｇ抗原性。
Experimental observation on efficacy of combination of methylene blue and light irratiation (methylene blue photodynamic method) in inactivating the viruses in human plasma in vitro was carried out in laboratory. Adding methylene blue 1 mol/L to human plasma and irradiation with the light from bromine tungsten lamp at 10 000 lx intensity for 5 min could inactivate 1065TCID50of vesicular stomatitis virus (VSV) and 10741TCID50of Sindbis virus (SV). Exposure for 2 min could inactivate 105.09TCID50of HIV-1, while the inactivating efficacy of either methylene blue or light alone was very low. However, this combination with a 60 min exposure time had no obvious destructive effect on HBsAg antigenicity.
出处
《中国消毒学杂志》
CAS
1999年第2期65-68,共4页
Chinese Journal of Disinfection
关键词
亚甲蓝光敏法
血浆消毒
输血疗法
methylene blue photodynamic methodplasma disinfectionSindbis virus vesicular stomatitis virusHIV-1virus inactivationHBsAg
