摘要
报道用高效毛细管电泳技术快速测定内江猪血清头孢唑啉钠（ｃｅｆａｚｏｌｉｎｓｏｄｉｕｍ，ＣＥＺ）的方法。血清用乙腈去除蛋白质，经离心后上清直接进样，毛细管内径７５μｍ，有效长度４０ｃｍ，在５０ｍｍｏｌ／Ｌ碳酸钠／碳酸氢钠缓冲液（ｐＨ１０）中，电压２０ｋＶ分离５分钟，２５４ｎｍ紫外检测。此方法操作简单，测定迅速，线性范围为５～３２０μｇ／ｍｌ，日内相对标准偏差为１．７％，日间相对标准偏差为８．０％，平均加样回收率为１０７．９％，最低检测限为１．５μｇ／ｍｌ，定量灵敏度为３．６μｇ／ｍｌ。按１６ｍｇＣＥＺ／ｋｇ对６头成年内江猪作静脉注射，测定结果经药动学软件分析得到药代动力学参数，结果表明内江猪对ＣＥＺ的代谢为二室开放模型，清除率为８．４３ｍｌ／（ｋｇ·ｍｉｎ）。
A rapid high performance capillary electrophoretic method was established to assay cefazolin in porcine blood serum. Porcine serum was deproteined by 60% acetonitrile and superlants were injected with 10s pressure load. The final assay condition was: 40cm75m silica capillary, 50mmol/L bicarbonate buffer at pH10, 20kV for 5min, 254 nm UV detection. The assay was linear within the range of 5320g/ml, the intraday and interday relative standard deviations ranged from 1.7% to 8.0%. Analytical recovery of cefazolin was 107.9%, the limit of detection was 1.5g/ml, the limit of quantitation was 3.6g/ml. To investigate the pharmacokinetic profile of cefazolin in pig, six adult pigs were injected with 16mg/kg cefazolin and all data was analyzed by pharmacokinetic software 3P87. Cefazolin metabolism in adult pigs appeared to be a typical twocompartment model with a clearance of 8.43ml/(kgmin).
出处
《华西医科大学学报》
CSCD
1999年第2期120-122,共3页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
四川省重大科技攻关项目资助
关键词
头孢唑啉
高效毛细管电泳
血药浓度
CefazolinHigh performance capillary electrophoresisDrug concentration in porcine serum
