摘要
目的探讨胆石病患者缩胆囊素（ＣＣＫＡ）受体基因表达及其与胆囊排空功能的关系。方法２０例单纯胆囊结石病患者和１０例健康正常人，术前用Ｂ超测定胆囊排空功能，另以８例因意外死亡无胆囊结石患者胆囊标本为测定ＣＣＫＡ受体基因表达的正常对照。ＣＣＫＡ受体ｍＲＮＡ表达的测定用ＲＴＰＣＲ方法。结果胆石病组胆囊排空功能明显异常。胆石病组ＣＣＫＡ受体ｍＲＮＡ表达量（０６３３±０１６７）明显低于对照组（０９４４±０２３０）（Ｐ＜００１）；在胆石病患者中，收缩减弱组的ｍＲＮＡ表达（０５４４±０１２４）又明显低于收缩正常组（０７６８±０１３１）（Ｐ＜００５），且ＣＣＫＡ受体ｍＲＮＡ表达的变化与胆囊排空率呈显著正相关（ｒ＝０９２５，Ｐ＜００１）。结论胆石病患者胆囊ＣＣＫＡ受体ｍＲＮＡ表达降低，此降低的基因表达与胆石病胆囊排空功能损害有关。
Objective To
investigate the expression of CCKA receptor and its relation with gallbladder hypomotility in
patients with gallstone. Methods 20 patients with gallstone and 10 normal subjects were
studied and gallbladder emptying function was measured by B ultrasonography. The other 8
patients without gallstone who died of accident were selected as controls for measuring mRNA
expression of CCKA receptor of gallbladder; RTPCR was used to explore gene expression of
CCKA receptor of gallbladder. Results The gallbladder motility was significantly impaired in
gallstone patients. The patients had lower mRNA expression of CCKA receptor than control
subjects(06330167 vs. 09440230, P<001), and so did petients with impaired galltb ladde
motility then those with normal motility(05440124 vs. 07680131,P<005). Furthermore, the
change of expression of CCKA receptors was markedly correlated with gallladder
emptying(r=0925,P<001).T5HZConclusions mRNA expression of CCKA receptor in gallstone
patients decreases, which was related to galbadder hypomotility. The downregulation of gene
expression of CCKA receptor plays an importent role in gallbladder hypomotility in patients with
gallstone.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第5期292-294,共3页
Chinese Journal of Surgery
基金
上海市高教局博士点基金
关键词
胆囊结石
胆囊收缩素受体
基因表达
胆囊排空
GallstoneReceptor,cholecystokininGene expressionGallbladder motility
