摘要
目的观察烫伤后机体主要脏器脂多糖受体ＣＤ１４（ＣＤ１４）和肿瘤坏死因子α（ＴＮＦα）ｍＲＮＡ表达的变化规律及二者的相互关系。方法采用大鼠３５％Ⅲ度烫伤模型，分别于伤前、伤后１２、２４、４８、７２小时活杀动物。留取组织标本或分离腹腔巨噬细胞，检测组织或巨噬细胞ＣＤ１４、ＴＮＦαｍＲＮＡ表达。结果烫伤后肝、肺、肾、肠等组织ＣＤ１４ｍＲＮＡ水平均有不同程度增高，分别于伤后１２小时和４８小时出现两个高峰。并且伤后腹腔巨噬细胞ＣＤ１４ｍＲＮＡ表达也显著增强。烫伤后各组织ＴＮＦα基因表达均有所增强。肝、肺、肾组织ＣＤ１４ｍＲＮＡ与ＴＮＦαｍＲＮＡ呈显著正相关（Ｐ＜００１）。结论烫伤打击可能上调机体主要脏器ＣＤ１４ｍＲＮＡ表达，并激活多种组织ＴＮＦα的基因表达。二者相互作用在介导内毒素所致多脏器损害中可能具有一定作用。
Objective To
investigate changes in lipopolysaccharide receptor CD14 and tumor necrosis factor (TNF) gene
expression in vital organs, and their significance in the pathogenesis of systemic inflammation
after burns. Methods Wistar rats were subjected to a 35 percent fullthickness scald injury, then
sacrificed preburn, and postburn 12, 24, 48, 72 hours, respectively. Tissue samples from liver,
kidney, lung and intestine were collected to measure CD14 and TNF mRNA expression.
Peritoneal macrophages were harvested by peritoneal lavage to determine CD14 mRNA
expression. Results CD14 mRNA expression increased markedly after thermal injury, peaking
at both 12 and 48 hours. Likewise, CD14 mRNA levels were significantly upregulated in
peritoneal macrophages. Gene expression of TNF elevated markedly in liver, lungs, and
kidneys after acute insults. Positive correlation was found between CD14 mRNA and TNF mRNA
in liver,lung, and kidney tissues. Conclusions5BZThermal injury per se can markedly
upregulate both CD14 and TNF gene expression in various organs. Excessive CD14 mRNA
expression may enhance synthesis and release of TNF stimulated by endotoxin translocation,
and the interaction between CD14 and TNF may play an important role in mediating multiple
organ damage secondary to major burns.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第5期271-273,共3页
Chinese Journal of Surgery
基金
军队杰出中青年人才专项基金
全军"九五"医药卫生科研基金
中国工程科技奖助基金
