摘要
目的 观察烫伤后肺组织脂多糖受体CD14基因表达的变化规律及其与急性肺损伤发生、发展的关系。 方法 采用大鼠 35 %体表总面积Ⅲ度烫伤模型 ,分别于伤前、伤后 12 ,2 4,48,72h处死大鼠。留取肺组织标本或分离腹腔巨噬细胞 ,分别检测组织内毒素含量、CD14mRNA表达及组织髓过氧化物酶 (MPO)活性。 结果 烫伤后肺组织内毒素含量和MPO活性均明显增高 ,且一直持续至伤后 72h。相关分析显示 ,肺组织内毒素含量与MPO活性呈显著正相关 (P <0 .0 5 )。另一方面 ,烫伤后肺组织CD14mRNA水平明显增高 ,分别于伤后 12 ,48h出现两个高峰。并且伤后腹腔巨噬细胞CD14mRNA表达也显著增强。 结论 烫伤后移位内毒素聚集于肺脏 ,可明显刺激机体局部组织CD14mRNA的表达 ,而组织CD14表达上调与严重烫伤后急性肺损伤的发生。
Objective To investigate changes of lipopolysaccharide receptor CD14 gene expression in pulmonary tissues and its significance in the pathogenesis of acute lung injury. Methods Wistar rats were subjected to a 35% of grade Ⅲ scald, then sacrificed afer scald at 12, 24, 48, 72 hours, respectively. Tissue samples from lungs were collected to measure tissue endotoxin levels, CD14 gene expression and pulmonary myeloperoxidase (MPO) activities. Peritoneal macrophages were also harvested by peritoneal lavage to determine CD14 mRNA expression. Results It was found that pulmonary endotoxin levels and MPO activities increased markedly after scald, and remained marked elevation for 72 hours from scald, Additionally, there was high positive correlation between pulmonary endotoxin levels and MPO activities. On the other hand, CD14 mRNA expression increased markedly after scald, peaking at 12 and 48 hours respectively. Likewise, CD14 mRNA levels were significantly up-regulated in peritoneal macrophages. Conclusions Endotoxin accumulated in local sites can markedly enhance CD14 mRNA expression in lungs. Up-regulation of pulmonary CD14 expression is associated with the development of lung injury following scald.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2000年第7期408-410,共3页
Chinese Journal of Trauma
基金
国家重点基础研究发展规划资助项目!(G1999054203)
国家自然科学基金资助项目!(39870286)
军队杰出中青年人才专项基金资助项目!(98J
