摘要
目的观察翡翠贻贝多糖(PVPS)对体外培养人乳腺癌MCF-7细胞增殖的抑制作用。方法体外培养的乳腺癌MCF-7细胞经不同浓度翡翠贻贝多糖处理48h后,经CCK-8试剂盒检测分析细胞存活率、用流式细胞术分析各组细胞凋亡率;光镜下观察40mg/L翡翠贻贝多糖处理细胞不同时间后的细胞形态变化。结果翡翠贻贝多糖40mg/L组和80mg/L组的乳腺癌细胞存活率明显低于对照组,而细胞凋亡率则明显高于对照组(P<0.05或P<0.01)。光镜下可见肿瘤细胞脱落随作用时间延长而明显。结论翡翠贻贝多糖可抑制体外培养乳腺癌细胞增殖并诱导其凋亡。
Objective To investigate the exprimental study on the proliferation of human breast cancer MCF-7 cell restrained by Perna viridis polysaccharide（PVPS） in vitro.Methods The Experiment study on the proliferation of human breast cancer MCF-7 cell handled 48 h by PVPS was assessed using CCK-8 method.The apoptosis of breast cancer cell was assessed using flow cytometry method.Observe the morphological changes of breast cancer cell by optical microscope.Results The breast cancer MCF-7 cell proliferation decreased and cell apoptosis increased induced by PVPS（at 40 mg/L,80 mg/L）（P0.05 or P0.01）.The shedding of breast cancer cell was proportional to the action time.Conclusion The Perna viridis polysaccharide can inhibit the proliferation and induce apoptosis of human breast cancer MCF-7 cell in vitro.
出处
《中国现代药物应用》
2011年第1期17-18,共2页
Chinese Journal of Modern Drug Application
基金
广东省自然科学基金(项目编号:5011588)
湛江市科技攻关项目(2008C03015)
关键词
翡翠贻贝
多糖
乳腺癌
细胞增殖
Perna viridis
polysaccharide
breast cancer
cell proliferation
