摘要
研究提取淡水鱼精巢中的核蛋白和鱼精蛋白的工艺条件。采用正交试验法。通过筛选，确定了提取核蛋白的最佳工艺条件为：NaCl浓度为1．0mol／L，NaCl用量为10倍，提取时间1h，95％乙醇用量为2倍。提取鱼精蛋白的最佳工艺条件为：H2SO4浓度为5．0％，H2SO4用量为3倍，提取时间2h，95％乙醇用量为3倍。
The extraction process of nucleoprotein and protamine from freshwater fish spermary was studied.The extraction conditions were optimized by using the orthogonal design.In extracting nucleoprotein,NaCl concentration was 1.0mol/L,the volume of NaCl solution was ten times as large as that of fish spermary,extraction time was 1 hour,the volume of 95% alcohol solution was two times as large as that of fish spermary.In extracting protamine,H2SO4 concentration was 5.0%,the volume of H2SO4 and 95% alcohol solution were three times as large as those of fish spermary,extraction time was two hours.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1999年第7期30-33,共4页
Food Science
关键词
淡水鱼
精巢
核蛋白
鱼精蛋白
提取工艺
Freshwater fish spermary Nucleoprotein protamine The conditions of extraction technology
