摘要
利用同源性克隆方法,从烤烟栽培品种K326(Nicotiana tobacumK326)中克隆获得烤烟八氢番茄红素合成酶基因的全长cDNA序列(命名为T-psy1),GenBank中的登录号为HM345582,该基因全长为1521bp,编码的蛋白含441个氨基酸,蛋白登录号为ADK25054。Blast搜索结果及进化分析结果表明,该基因序列与观赏烟草、番茄和辣椒的八氢番茄红素合成酶基因同源性分别为95%,91%,86%。其编码蛋白与观赏烟草、番茄、牛奶子的PSY基因编码蛋白一致率分别为97%,90%和74%。
Adopting a homology cloning method, the full length cDNA sequence of tobacco phytoene synthase gene （named T-psyl） was cloned from flue-cured tobacco cv. K326, the T-psyl gene, GenBank accession number HM345582, included 1521bp, with coding protein containing 441 amino acids, protein accession number ADK25054. The results of BLAST search and phylogenetie analysis showed that T-psyl presented high genetic homologies with the phytoene synthase of ornamental tobacco, tomato and pepper, which were 95% , 91% and 86% , respectively; and the coding protein consistences of T-psyl gene with phytoene synthase gene of ornamental tobacco, tomato and Elaeagnus umbellate were 97% , 90% and 74% , respectively.
出处
《烟草科技》
EI
CAS
北大核心
2011年第2期58-63,共6页
Tobacco Science & Technology
基金
贵州省烟草专卖局资助项目"分子生物技术在烤烟育种上的应用基础研究"(黔烟科2007-03)
"烟草病圃微生物多样性分析及烟草抗性基因研究"(黔烟科2009-15)
