摘要
目的:建立测定康泰胶囊(防风、白芍、木香)含量的方法。方法:采用高效液相色谱法在同一色谱条件[色谱柱:Kroma-sil 100-5C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液梯度洗脱,流速1.0 mL.min-1;柱温:30℃;检测波长:220 nm]下对制剂中升麻苷、芍药苷、5-O-甲基维斯阿米醇苷、木香烃内酯及去氢木香烃内酯的含量进行测定。结果:升麻苷、芍药苷、5-O-甲基维斯阿米醇苷、木香烃内酯、去氢木香烃内酯的线性范围分别为13.15～236.74 ng(r=0.9999),246.00～4428.00 ng(r=0.9995),35.12～632.16 ng(r=0.9993),35.00～630.00 ng(r=0.9997),27.20～489.60 ng(r=0.9996);平均回收率(n=9)分别为101.0%,100.9%,99.79%,101.0%,100.2%。结论:该方法简便、快速、准确,重现性好,可作为康泰胶囊质量控制的方法。
Objective：To establish a method for the simultaneous determination of prim-O-glucosylcimifugin,paeoniflorin,5-O-methylvisammioside,costunolide and dehydrocostuslactone in Kangtai capsules.Methods：The HPLC system consisted of a Kromasil 100-5C18（250 mm×4.6 mm,5 μm） column,acetonitrile-0.1% phosphoric acid as mobile phase with gradient elution at a flow rate of 1.0 mL·min-1,and detection wavelength of 220 nm.Results：The linear ranges of prim-O-glucosylcimifugin,paeoniflorin,5-O-methylvisammioside,costunolide and dehydrocostuslactone were 13.15-236.74 ng（r=0.9999）,246.00-4428.00 ng（r=0.9995）,35.12-632.16 ng（r=0.9993）,35.00-630.00 ng（r=0.9997）,27.20-489.60 ng（r=0.9996）,respectively;The average recoveries（n=9） were 101.0%,100.9%,99.79%,101.0%,100.2%,respectively.Conclusion：The method is simple,quick,accurate and reproducible,and can be used for the quality control of Kangtai capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第2期240-243,共4页
Chinese Journal of Pharmaceutical Analysis
