摘要
目的:探讨负载胰腺癌抗原的树突状细胞(DC)疫苗对胰腺癌患者免疫功能的影响。方法:从胰腺癌患者外周血单个核细胞中诱导DC,并用重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和白细胞介素4(rhIL-4)刺激活化,经热休克凋亡胰腺癌细胞致敏制备DC疫苗。将30例胰腺癌患者随机分为化疗加用DC瘤苗治疗(A)组15例,单纯化疗对照(B)组15例。对两组患者治疗前后T细胞亚群及自然杀伤(NK)细胞及血清IL-2、IL-12、γ干扰素(IFN-γ)水平进行比较,并进行迟发性超敏反应(DTH)试验。结果:A组治疗后外周血CD3+、CD3+CD4+、CD4+/CD8+及NK细胞比率较治疗前明显升高(P<0.05),且明显高于B组(P<0.05);A组治疗后血清IL-2、IL-12、IFN-γ水平较治疗前明显升高(P<0.05),A组中有4例患者DTH试验呈阳性。结论:负载胰腺癌抗原的DC疫苗治疗可改善患者的免疫功能。
Objective：To investigate the effects of dendritic cells（DCs） loaded with pancreatic cancer cells antigen on the immune function of the patients with unresectable pancreatic cancer.Methods：DCs isolated from peripheral blood mononuclear cell of pancreatic cancer patients were cultured and stimulated using rhGM-CSF and rhIL-4 in vitro,and then were pulsed with pancreatic cancer cells antigen.Thirty patients with unresectable pancreatic cancer were randomly divided into 2 groups.The dendritic cell vaccine was used in 15 cases （group A）,and chemotherapy was applied in 15 cases （group B） as controls.Immune function and clinical effects were compared between the two groups.Results：The levels of CD3＋、CD3＋CD4＋、CD4＋/CD8＋ and NK cell in group A were increased after vaccination（P 0.05）.So did the levels of IL-2,IL-12,IFN-γ（P 0.05）.Conclusion：The DCs vaccines loaded with pancreatic cancer cells antigen could improve the immune function of the patients with unresectable pancreatic cancer.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第11期1649-1652,共4页
Journal of Nanjing Medical University(Natural Sciences)
关键词
树突状细胞
胰腺癌
免疫治疗
dendritic cells
pancreatic cancer
immunotherapy
