摘要
目的通过对sD大鼠脑外伤合并四肢骨折不同时间段骨痂局部转化生长因子（TGF-β）的基因表达进行检测，了解TGF-β在增强骨折愈合过程中的作用。方法纯种SD雄性大鼠80只，随机分为（每组40只）单纯四肢骨折组（对照组）与脑外伤合并四肢骨折组（实验组）（每组40只），两组均利用逆转录PCR基因扩增技术对骨折后1、2、3、4周骨痂局部TGF-β基因表达的改变进行检测。结果与对照组相比，实验组在骨折后1周TGF-β的基因表达明显升高，差异有统计学意义（P〈0．05）；在骨折后3周其表达明显下降，差异有统计学意义（P〈0．05）；两组TGF-β基因表达均在骨折后2周达到峰值，峰值与其他时间段比较差异均有统计学意义（P〈0．05），但两组间峰值比较差异无统计学意义（P〉0．05）。结论脑外伤合并四肢骨折骨痂局部TGF-β基因表达模式的改变表明，TGF-β基因参与了增强的骨折愈合病理生理过程。
Objective To investigate gene expression of transforming growth factor-β（TGF-β） in local bony callus in traumatic brain injury combined with extremity long bone fracture in rats. Methods Eighty male SD rats were randomized into 2 even groups： the experimental group were models of traumatic brain injury combined with extremity bone fracture and the control group were models of simple extremity bone fracture. Samples of bony callus were harvested at 1, 2, 3, and 4 weeks in both groups, each time from 10 rats respectively, for detection of gene expressions of TGF-β by reverse transcription PCR techniques. Results Levels of gene expression of TGF-β in local bony callus in the experimental group were significantly higher than in the control group at one week （ P 〈 0. 05）; but significantly lower at 3 weeks（ P 〈 0.05） . Peak values occurred at 2 week in both groups, though, significantly higher than at other times, there was no significant difference between the 2 groups （ P 〉 0. 05） . Conclusion Changes in gene expression of TGF-β in local bony callus in traumatic brain injury combined with extremity long bone fracture indicate that TGF-β may play a role in the process of increased fracture healing.
出处
《中华创伤骨科杂志》
CAS
CSCD
2011年第2期175-177,共3页
Chinese Journal of Orthopaedic Trauma
基金
基金项目：广东省科技厅科研基金（20088030301283）
广东省卫生厅医学科研基金（A2008609）
关键词
脑损伤
创伤和损伤
骨折
骨痂
基因表达
Brain injuries
Wounds and injuries
Fractures
Bony callus
Gene expression
