摘要
背景:研究表明,血管内皮细胞生长因子及碱性成纤维细胞生长因子均在软骨内成骨及骨折愈合血管增生过程中发挥重要作用。目的:对比观察血管内皮细胞生长因子、碱性成纤维细胞生长因子在骨折愈合过程中的作用。设计、时间及地点:以动物为观察对象,双因素设计的验证性实验,于2005-08/2006-05在华北煤炭医学院病理学实验室完成。材料:选用成年健康日本大耳白兔24只,共取兔双前肢桡骨48侧,制作双侧桡骨中段骨折动物模型。按随机数字表法分为内皮细胞生长因子、碱性成纤维细胞生长因子组,每组24侧。方法:内皮细胞生长因子组、碱性成纤维细胞生长因子组分别在骨折局部注射内皮细胞生长因子0.2μg,碱性成纤维细胞生长因子100ng,术后不用外固定。主要观察指标:分别于术后2,4和6周取材,测定骨痂矢状径、横径及截面积;通过X射线片观察骨折愈合情况,并测定外骨痂总面积;观察骨折愈合的组织学变化,并测定骨痂中小梁骨、软骨、纤维组织所占百分比。结果:①骨折后2周,内皮细胞生长因子组的骨痂横径及矢状径大于碱性成纤维细胞生长因子组(P<0.05);4周时碱性成纤维细胞生长因子组的骨痂矢状径及截面积大于内皮细胞生长因子组(P<0.01);6周时碱性成纤维细胞生长因子组的骨痂截面积大于内皮细胞生长因子组(P<0.01)。②骨折后2周,在X射线片上内皮细胞生长因子组可见较多外骨痂,骨折线较模糊;碱性成纤维细胞生长因子组骨折间隙较小。4周时碱性成纤维细胞生长因子组骨折基本愈合。6周时碱性成纤维细胞生长因子组骨折愈合。③骨折后2周,内皮细胞生长因子组组织切片小梁骨痂所占百分比大于碱性成纤维细胞生长因子组(P<0.01);6周时碱性成纤维细胞生长因子组的小梁骨痂所占百分比大于内皮细胞生长因子组(P<0.01)。结论:骨折时局部应用内皮细胞生长因子、碱性成纤维细胞生长因子可促进骨折愈合,内皮细胞生长因子促进早期骨折愈合,碱性成纤维细胞生长因子促进中晚期骨折愈合。
BACKGROUND： Studies have demonstrated that vascular endothelial cell growth factor （VEGF） and basic fibroblast growth factor （bFGF） play important roles in entochondrostosis and fracture healing blood vessel hyoperplasia. OBJECTIVE： To observe and compare the roles of VEGF and bFGF in fracture healing. DESIGN, TIME AND SETTING： Double-factor trial was performed at Pathological Laboratory, North China Coal Medical University from August 2005 to May 2006. MATERIALS： Twenty-four adult healthy Japan rabbits were selected and radial fracture models were created in 48 bilateral anterior limbs. The rabbits were randomly divided into VEGF group and bFGF group （n=24）. METHODS： VEGF （0.2 u g） and bFGF （100 ng） were injected respectively in the fracture site of rabbits. No external fixation was used. MAIN OUTCOME MEASURES： The rabbits were executed at postoperative weeks 2, 4 and 6 to measure callus sagittal diameter, transverse diameter and section area. X-ray was used to observe fracture healing and measure bony callus total area. Histological alterations during fracture healing were observed, and percentage of trabecular bone, cartilage and fibrous tissue in the bony callus was determined. RESULTS： At 2 weeks after operation, callus sagittal diameter and transverse diameter in VEGF group were significantly larger than bFGF group （P 〈 0.05）; at 4 weeks, callus sagittal diameter and section area in bFGF group were significantly larger than VEGF group （P 〈 0.01）; at 6 weeks, callus section area in bFGF group was significantly larger than VEGF group （P 〈 0.01）. At 2 weeks after bone fracture, there were lots of outside bony callus in VEGF group through X-ray films with unclear fracture line, while the space between the fractures in bFGF group was small. At 4 weeks, the fracture for group bFGF basically healed. At 6 weeks, the fracture healed in bFGF group. Two weeks after fracture, the trabecula bony callus percentage in VEGF group was significantly larger than bFGF group （P 〈 0.01）, while the bony callus percentage in bFGF group was significantly larger than VEGF group at 6 weeks （P 〈 0.01）. CONCLUSION： Local application of VEGF and bFGF can improve fracture healing. VEGF accelerates early fracture healing, and bFGF improve intermediate and advanced stage of fracture healing.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第33期6453-6456,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
