摘要
[目的]探讨西兰苔叶黄酮粗提物(EOF)的抗癌机制。[方法]通过四甲基偶氮唑盐(MTT)试验检测EOF对细胞生长的抑制率,并用流式细胞仪采用Annexi-V/PI双染法进行细胞凋亡检测,以探讨西兰苔叶EOF对癌细胞增殖的抑制作用及其诱导的癌细胞凋亡机制。[结果]西兰苔叶EOF对SW480、HepG2、Hela和A549这4种细胞的生长均表现出抑制作用,抑制率随药物浓度的增加而增大,呈明显的量效关系,其50%抑制浓度(IC50)值分别为88.14、99.65、104.43、79.77μg/ml,对SW480细胞的作用最为明显。通过Annexi-V/PI双染法流式细胞仪测得其浓度为30、60、80、120、160μg/ml时,诱导的SW480细胞的早期凋亡率分别为12.74%、16.41%、19.61%、28.28%和50.66%。[结论]西兰苔叶EOF是通过抑制增殖和诱导细胞凋亡来发挥对肿瘤的治疗作用的。
To investigate the mechanism of EOF on cell proliferation and apoptosis of human cancer cell line in vitro.The cell growth inhibiting ratio was assessed by the MTT assay,the cell apoptotic was detected with Annexi-V/PI doubled staining flow cytometry（FCM）.Human cancer cells line SW480、HepG2、Hela、A549 were treated with different concentrations of EOF respectively.MTT assay showed EOF inhibited the proliferation of human cancer cell line in a dose-dependent manner（P0.01）.The MTT assay results showed that the IC50 of EOF for SW480、HepG2 、Hela、A549 1were 88.14、99.65、104.43、79.77 μg/ml respectively.Compared with the control group,the early apoptosis rate in SW480 cells was found to be 12.74%、16.41 %、19.61%、28.28%、50.66%.Experimental results show that the EOF plays a role in tumor therapy by inhibiting proliferation and inducing apoptosis.
出处
《安徽农业科学》
CAS
北大核心
2010年第35期20014-20015,20024,共3页
Journal of Anhui Agricultural Sciences
