摘要
目的观察扶正抑瘤方对肝癌细胞增殖和凋亡的影响。方法采用体外培养肝癌细胞株HepG2,分别用扶正抑瘤方含药血清高中低剂量组和空白血清组干预。MTT法检测细胞增殖,流式细胞术检测细胞周期和细胞凋亡率。结果扶正抑瘤方含药血清组与空白血清组比较,OD值明显降低(P<0.05),S期细胞含量明显减少(P<0.01),Annexin V+/PI-区域和Annexin V+/PI+区域细胞含量不同程度增多(P<0.05或P<0.01),以高剂量含药血清组作用最为明显。结论扶正抑瘤方含药血清通过干扰肝癌细胞株HepG2 S期的合成有效抑制肝癌细胞的生长,同时诱导肝癌细胞株HepG2的早期和晚期凋亡,清除增殖过度的肝癌细胞。
Objective To observe the effect of Fuzheng Yiliu decoction on proliferation and apoptosis of hepatoma cells.Methods Hepatoma HepG2 cells were cultured in vitro and treated with Fuzheng Yiliu decoction drug-containing serum with different doses,blank serum respectively,then proliferation rate was detected with methyl thiazolyl tetrazolium assay,and cell cycle and apoptosis rate were detected with flow cytometry.Results Compared with blank serum group,the OD value in drug-containing serum groups were decreased obviously（P0.05）,and S phase cells were also decreased obviously（P0.01）.The cells increased in different degree in Annexin V＋/ PI-region and Annexin V＋/ PI＋ region（P0.05 or P0.01）,and the increasing amplitude of cells in drug-containing serum group with high doses was most obvious.Conclusion Fuzheng Yiliu decoction drug-containing serum could effectively inhibit the growth of hepatoma cells by interfering the synthesis of HepG2 cells in S phase,induce early and late apoptosis of HepG2 cells,and remove the hepatoma cells which have proliferated excessively.
出处
《福建中医药大学学报》
2010年第4期30-32,35,共4页
Journal of Fujian College of Traditional Chinese Medicine
基金
国家科技部国际科技合作项目(2008DFA32200)
福建省自然科学基金(2009J01170)
陈可冀中西医结合发展基金资助项目(CKJ2008002)
关键词
扶正抑瘤方
肝癌
细胞周期
凋亡
含药血清
Fuzheng Yiliu decoction
hepatoma
cell cycle
apoptosis
drug-containing serum
