摘要
以橡胶树热研88-13品种的幼嫩种子的珠心组织为材料,诱导出胚性愈伤组织。对胚性愈伤组织诱导培养基、继代培养基中不同的影响因素如植物生长调节剂的种类和浓度、钙离子浓度等进行了研究,筛选到了合适的影响因素。经过连续5个月的继代选择培养,逐渐诱导出易碎的胚性愈伤组织。对易碎胚性愈伤组织进行了长期继代培养。组织学切片证明长期继代培养的愈伤组织维持了胚性的状态。取继代培养了2年多的橡胶树热研88-13品种的珠心易碎胚性愈伤组织诱导胚状体,得到了186个胚状体,正在诱导植株再生。
The nucellar tissue from the young seeds of rubber tree clone Reyan 88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations in embryogenic callus induction media and subculture media had been analyzed and the optimal culture parameters had been determined. Through continuous subculture and selection for 5 months,friable embryogenic callus had been gradually obtained. Long term subculture had been carried on for the friable embryogenic calli. Histological sections proved that the calli proliferated by long term subculture maintained the embryogenic characters. The nucellar friable and embryogenic calli of rubber tree clone Reyan 88-13 that had been subcultured for more than 2 years were used for inducing embryoids,and 186 embryoids were obtained,which were being used to induce plant regeneration.
出处
《热带生物学报》
2010年第4期307-313,共7页
Journal of Tropical Biology
基金
国家自然科学基金项目(30760128)
科技部国际科技合作项目(2008DFA32020)
海南省重点科技项目(070105)
行业科技专项子课题(nyhyzx07-033-6)
基本科研业务费专项资金项目(XJSY-WFZX2009-01)
农业部"948"项目(2010-S7)
关键词
橡胶树
珠心
易碎胚性愈伤组织
长期继代培养
胚状体
Hevea brasiliensis Mll. Arg.
nucellus
friable embryogenic callus
long-term subculture
embryoid
