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遗传转移的MADI过程 被引量:20

THE MADI PROCESS FOR GENETIC TRANSFER
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摘要 单体附加在小麦细胞中的黑麦染色体,在减数分裂过程中高频率地断裂,破碎和丢失。它们同时引起小麦染色体的断裂和丢失。断裂的黑麦和小麦染色体,以较高频率重新融合形成罗伯逊易位。破碎的黑麦染色体的DNA片段,可以整合在小麦染色体上,完成遗传的转移。本研究发现的这种通过染色体的“单体附加一破碎一整合”过程实现遗传转移的方式,简称为“MADI”(美代)过程,为把外源种质导入栽培植物的研究提供了一种有效的方法。 The monsomie addition chromosomes of rye in wheat were eliminated rapidly upon selfing due to their highly frequent breakage and disruption in meiosis. The added rye chromosomes, in varying number, affected significantly the transmission rate of wheat chromomes and caused a remarkably high rate of Robertsonian translocation between wheat and rye chromosomes. The small DNA segments, which were produced by disruption of added rye chromosomes, can be incorporated in wheat chromosomes to accomplish transfer of the desired genes. This procedure for genetic transfer in the present study is named briefly'MADI' precess(Monosomic Addition-Disruption-Incorporation). The restlts indicate that this method allow for the effeetive transfer of genetic material from rye into common wheat.
作者 任正隆
出处 《四川农业大学学报》 CSCD 1990年第1期1-6,共6页 Journal of Sichuan Agricultural University
关键词 小麦 染色体 遗传 CHROMOSOME(S) HECEDIIABILITY WHEAT.
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参考文献2

  • 1Hoan T. Le,K. C. Armstrong,Brian Miki. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe[J] 1989,Plant Molecular Biology Reporter(2):150~158
  • 2Dr. A. J. Lukaszewski,J. P. Gustafson. Translocations and modifications of chromosomes in triticale × wheat hybrids[J] 1983,Theoretical and Applied Genetics(3):239~248

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