摘要
为探讨新鲜无花果叶超声提取物(fig leaf extract,FLE)对体外培养的人肝癌细胞株HepG2增殖和凋亡的影响,用不同浓度FLE处理肝癌HepG2细胞.采用细胞形态学观察、细胞活力测定(MTT法)来评价FLE对HepG2细胞体外增殖的影响,应用凋亡相关蛋白caspase3和p53免疫组织化学法及流式细胞术的膜联蛋白V/碘化丙啶法(Annexin V/PI法)来检测细胞凋亡情况.形态学观察发现FLE处理24 h细胞出现明显凋亡,细胞增殖活力显著降低(P<0.05或P<0.01),流式细胞仪检测到FLE处理12 h所有剂量组细胞平均凋亡率均高于对照组(P<0.05或P<0.01),免疫组化结果显示caspase3表达增强(P<0.05,P<0.01),p53蛋白表达减弱(P<0.05),以上作用效果呈现剂量依赖性.以上结果说明无花果叶提取物能够通过激活caspase3和p53诱导肝癌HepG2细胞凋亡从而抑制其体外的生长与增殖.
Different concentration of fig leaf extract（FLE） was added in culture fluid to illustrate its effect on the growth inhibition and apoptosis of human liver cancer HepG2 cells.Morphologic changes of gastric cells were observed under an inversion microscope,colony formation inhibition was used to reveal the effect of FLE on proliferation of HepG2 cells.Immunohistochemical staining detecting the expression of caspase3 and p53 were chose to detect the apoptosis of HepG2 cells induced by FLE,and flow cytometry was also used to determine the apoptosis of cells by Annexin V/PI assay.Morphologic alterations of cells were observed after treated with FLE for 24 h.Cell proliferation was inhibited by FLE（P〈 0.05）.Flow cytometry analysis revealed significantly increase in apoptosis cells contrast with control group after treatment with all doses of FLE for 12 hours（P〈 0.05 or P 0.01）.Immunohistochemical staining showed that the expression of caspase3 increased significantly（P 〈0.05,P〈 0.01） and the expression of p53 in the cells decreased significantly（P 〈0.05）.Fig leaf extract may inhibit the growth and proliferation of human liver cancer HepG2 cells and induce cell apoptosis through activing caspase3 and p53.
出处
《生命科学研究》
CAS
CSCD
2010年第6期523-527,共5页
Life Science Research
基金
山东省高等学校科技计划项目(J08LG77)
