摘要
采用引物BOX和ERIC对来自4省的35个细条病菌菌株进行Rep-PCR扩增,BOX引物扩增出14条指纹带,10种谱型,以致病型为单位计算遗传多样性值为0.63～1.00;ERIC引物扩增出19条指纹带,17种谱型,遗传多样性值为0.86～1;引物ERIC比BOX在遗传多样性方面有更好的分辨率;树状聚类图反映的遗传分簇差异,与菌株的致病型及其地理的来源之间没有相关性。
Rep-PCR was used to analyze the genetic diversity in a population of Xanthomonoas oryzae pv.oryzicola （Xoo） isolated from 4 provinces. Genomic DNA from 35 strains was amplified with two specific primers （BOX and ERIC）. 14 bands and 10 patterns of DNA fringer-prints were amplified by BOX-PCR. On the other hand, 19 bands and 17 patterns were gained by ERIC-PCR. The range of genetic diversity value was 0.63-1.00 with BOX- PCR and 0.86-1.00 with ERIC-PCR based on pathotypes, which indicated the primer ERIC was better than BOX in estimating the genetic diversity of Xoo. There was no correlation between the pathotypes, geographic source and clusters based on patterns of DNA fringerprints.
出处
《当代生态农业》
2010年第3期13-16,12,共5页
Contemporary Eco-Agri Culture
关键词
致病型
水稻细条病菌
遗传多样性
pathotype
Xanthomonas oryzae pv. Oryzicola
gentic diversity
