摘要
试用４０ 个引物对我国水稻条斑病菌、“稻短条斑病菌”和李氏禾条斑病菌等１４ 个代表菌株进行ＲＡＰＤ 分析，其中１１ 个引物的扩增产物表现明显的多态性， 共扩增出１５８ 条谱带，多态性为８９ ．７４ ％ 。聚类分析结果显示１４ 个菌株可区分为３ 个类群，第１ 群包括ＬＬＳ２ 、ＬＬＳ３ 、ＬＬＳ４ 、ＲＳ０５ 、Ｒ１００８ 、ＴＡＳ和ＴＡＸ；来自不同稻区水稻条斑病菌的群体结构差异明显，分属于第２ 群（ 如ＲＳＨａｉ 等） 和第３ 群（ 如ＲＳ１０５ 等） 。菌株ＤＮＡＲＡＰＤ 指纹分析和致病性测定结果证明：李氏禾条斑病菌、“稻短条斑病菌”在水稻和李氏禾上不仅可相互侵染，而且遗传背景的相似性较高，确认是同一种病菌，其与小麦黑颖病菌亲缘关系较近。
Genetic diversity of 14 pathogenic bacteria isolates of the ‘rice short streak’ and the rice and leersiae bacterial leaf streak were assessed by RAPD with 40 primers.Eleven primers revealed reproducible polymorphism and a total of 158 bands were scored.The frequency of genetic diversity was 89.74%.Results from comparative resarch of cluster analysis base on the RAPD bands of 14 isolates and their pathogenicity on rice suggested that the relationship between LLS2,LLS3,LLS4,RS05,R1008,TAS,and TAX were very close,belonging to the same group (I).Highest similarity was found between TAS and TAX.High genetic diversity was observed among isolates derived from rice bacterial leaf streak,belonging to two different groups (Ⅱ,Ⅲ).Results indicated that isolates LLS2,LLS3,LLS4,R1008,RS05 are closer to the isolates derived from rice(TAS,TAX)than to the others.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第2期120-125,共6页
Acta Phytopathologica Sinica
基金
中国农科院植保所国家重点实验室开放基金
关键词
稻短条斑病菌
水稻条斑病菌
DNA
多态性
rice short streak”,leaf streak, Xanthomonas oryzae pv. oryzicola , Xanthomonas leersiae ,RAPD
