摘要
目的 探讨慢性间质性肾炎发生发展中炎症细胞浸润与粘附因子表达的特点。方法 用雌性Ｗｉｓｔｅｒ 大鼠，皮下注射嘌呤霉素氨基核苷（ＰＡＮ） 建立慢性间质性肾炎动物模型。于注射后０ 、３ 、５ 、８、１２ 周分别检测２４ 小时尿蛋白定量，分批取出肾脏作ＨＥ染色，免疫组织化学ＡＢＣ法标记单克隆抗体ＣＤ４、ＣＤ８、ＭΦ、粘附分子１（ＩＣＡＭ１） 、ＣＤ１１ａ、ＣＤ１１ｂ、ＣＤ１８、ＣＤ４４ 、ＨＡ、ＴＣＲ（α，β）、ＭＨＣⅡ。结果 实验组第３ 周始２４ 小时尿蛋白量逐渐增加，与对照组比较有显著性差异，ＨＥ染色可见肾间质炎症细胞浸润，ＣＤ４ 阳性、ＴＣＲ阳性、ＩＣＡＭ１ 阳性细胞增多，第８ 周２４ 小时尿蛋白量达高峰，出现肾间质纤维化，肾小管萎缩、ＩＣＡＭ１、ＴＣＲ、ＣＤ４４、ＨＡ、ＣＤ１１ａ、ＣＤ１１ｂ、ＣＤ１８、ＭＨＣ Ⅱ阳性细胞数达高峰；第１２ 周，多种细胞因子表达及２４ 小时尿蛋白定量呈下降趋势。结论 ＣＤ４ 阳性细胞的浸润增殖，启动了多种细胞因子表达，在慢性间质肾炎发生发展中起重要作用。
Objective To investigate the characteristics of the infiltration of the inflammatory cells in the development of chronic interstitial nephritis.Methods PAN was injected subcutaneously to famale Wister rats to induce animal models of chronic intersitial nephritis.Proteinuria per 24 hours respectively in 0,3,5,8,and 12 weeks after the injection was measured.Kidneys in divided groups were taken out respectively,stained with HE and labeled the monoclonal antibodies such as CD4,CD8,MΦ,ICAM 1,CD11a,CD11b,CD18,HA,TCR(α,β),MHCⅡ with the immunohistochemical ABC technique.Resuls From the 3rd week after injection,the proteinuria per 24 hours increased gradually in experimental group.There was a significant difference compared with the control group.The infiltration of the inflammatory cells was found in renal interstitium by HE staining.The CD4,TCR and ICAM 1 positive cells incrcased at the same time.In the 8th week,the proteinuria per 24 hours reached the peak level.The interstitial fibrosis and the tubular atrophy appeared.ICAM 1,TCR,CD44,HA,CD11a,CD11b,CD18 and MHC Ⅱ positive cells reached the peak level.In the 12th week,the expression of varieties of cytokines and the quantity of the protein tended to decrease.Conclusion The infiltration and proliferation of CD4 positive cells may initiate the expression of varieties of cytokines which plays a key role in the development of chronic interstitial nephritis.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1999年第6期372-375,共4页
Chinese Journal of Nephrology
关键词
间质性肾炎
细胞因子
动物模型
T细胞
Interstitial nephritis Cytokine Animal model T lymphocyte
