摘要
通过PCR的方法对海洋噬菌体EJ(3)9P1中ORF172基因进行克隆,并构建了表达载体pET28-ORF172,转入大肠杆菌BL21,在IPTG诱导下进行表达,并通过SDS-PAGE对表达结果进行分析。结果成功构建了ORF172的表达体系,为进一步开展噬菌体EJ(3)9P1的尾丝基因结构与功能研究奠定基础。
The gene ORF172 of EJ（3）9P1,a marine bacteriophase was cloned by PCR and inserted into the prokaryotic expression vector pET-28a（＋）. The recombinant plasmid pET28-ORF172 was transformed into the BL21 cell to express the protein by induction with IPTG. The result showed that the system of the ORF172＇s expressing had been established. This study provides a useful tool that will prove its efficacy in the studies of gene function of the bacteriophase tail＇s genes.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2010年第6期1253-1256,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家高技术研究发展计划(863计划)资助项目(2006AA09Z420)
