摘要
研究了脂多糖(lipopolysaccharide,LPS)诱导草地贪夜蛾卵细胞系SF-9产生抗菌肽的过程.以三氯醋酸提取抗菌肽,抑菌圈法测定抗菌活性,结果显示形成了明显的抑菌圈;体外实验证实,提取的抗菌活性物质能显著抑制人宫颈癌细胞SiHa的生长;体内实验利用已建立的荷人宫颈癌细胞SiHa裸鼠模型,通过局部注射途径进行治疗干预实验,结果显示治疗组与对照组比较肿瘤明显缩小,有显著差异(P<0.01).研究结果表明昆虫抗菌肽具有较明显的抗瘤作用.
Spodoptera frugiperda egg cell line（SF-9） were induced with LPS.The insectival antibacterial peptides were recovered by trichloroacetic acid precipitation method,and the antibacterial activity was determined using the zone inhibition assay.They generated obvious inhibition zone.In vitro,antibacterial peptides can inhibit the proliferation of human cervical cancer cell line SiHa.An in vivo analysis in nude mice with tumor xenografts was established to conduct interference treatment.Antibacterial peptides were injected intratumorally.The treatment groups showed marked difference from the control group（P0.01）.Overall,these results indicated that the insectival antibacterial peptides were produced and possesses the potential to be a novel therapeutic agent for treating cervical cancer.
出处
《武汉工程大学学报》
CAS
2010年第12期39-42,共4页
Journal of Wuhan Institute of Technology
关键词
昆虫抗菌肽
裸小鼠
宫颈癌
SiHa细胞株
insectival antibacterial peptides
Nude mice
human cervical cancer
SiHa
