摘要
目的了解壳聚糖(CS)-明胶(Gel)/β-磷酸三钙(β-TCP)复合体支架超微结构、机械性能及生物相容性,探讨其作为组织工程软骨支架材料的可行性。方法采用二次冻干技术制备CS-Gel/β-TCP复合体支架,扫描电镜观察超微形态,材料万能测试机测定压缩强度和压缩模量;将CS-Gel/β-TCP复合体支架植入兔皮下,观察体内降解情况及生物相容性。结果混合溶液固含量、β-TCP添加量、预冻温度对复合体支架的孔隙结构具有决定性作用。β-TCP的引入能显著增强CS-Gel聚合体支架的机械性,随着β-TCP添加量的增多,复合体支架的压缩强度、模量都有较大程度提高;随着预冻温度的降低,材料的机械性能亦随之降低。在植入早期可观察到一过性炎性反应,随着植入时间延长,支架逐渐降解,12周时基本降解吸收。结论 CS-Gel/β-TCP复合体支架具有良好的超微结构、机械性能和生物相容性,是一种较好的构建组织工程软骨的支架材料。
Objective To investigate the ultra-microstructure,physical characteristics and biocompatibility of the man-made porous chitosan（CS）-gelatin（Gel）/β-tricalcium（β-TCP） complex,so as to explore the feasibility of the complex as cartilage tissue engineering scaffolds.Methods The CS-Gel/β-TCP sponges were prepared by freezing and lyophilizing taking water as the hole-making agent.The ultra-microstructure was observed with scanning electron microscope.The compress strength and modulus were determined by omnipotent material-testing machine.The complex was subcutaneously imbedded in the rabbit to observe the biodegradability and biocompatibility.Results The solid content of the mixed solution,the affix quantity of β-TCP and the preliminary freeze temperature play the decisive role of the hole structure of the scaffold complex.The compress strength and modulus of the scaffold improved significantly with the affix quantity of β-TCP increased.And the mechanical function reduced when preliminary freeze temperature lowered.Immunological rejection was observed on early stage after embedded.Then the scaffold degraded,and was absorbed at 12 weeks.Conclusion The CS-Gel/β-TCP complex scaffold has a 3-D network structure with high porosity,good mechanical function and good biocompatibility.It may be regarded as one of the important cartilage tissue engineering scaffolds with latent value.
出处
《组织工程与重建外科杂志》
2010年第6期319-322,共4页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
组织工程
支架材料
壳聚糖
明胶
Β-磷酸三钙
机械性能
生物相容性
Tissue engineering
Scaffold
Chitosan
Gelatin
Beta-tricalcium phosphate
Mechanical function
Biocompatibility
