摘要
目的在低氧条件下联合培养兔骨髓基质干细胞(Bone Marrow Stromal Cell,BMSC)及由其诱导而来的内皮细胞(Endothelial Cell,EC),观察并探讨低氧状态及EC对BMSC增殖的影响。方法从兔骨髓中分离获得BMSC,体外培养扩增后取部分向EC诱导。实验分4组:①BMSC常氧培养组;②BMSC低氧培养组;③BMSC+EC常氧联合培养组;④BMSC+EC低氧联合培养组。通过观察细胞的形态、增殖能力等了解低氧条件及EC对BMSC生长的影响。结果 BMSC可诱导为EC,EC与BMSC混合生长良好。MTT法检测显示,低氧培养组的细胞增殖能力明显高于常氧培养组(P<0.01),但相同条件下的BMSC单独培养组和BMSC+EC联合培养组间的细胞增殖能力没有显著性差异(P>0.05)。结论在一定时间内,低氧培养可以促进BMSC的增殖;BMSC来源的EC不能促进BMSC的增殖。
Objective To investigate the influence of hypnxia and co-culture of endothelial cells （EC） induced from BMSC and autologous bone marrow stromal cells （BMSC） on the proliferation of BMSC. Methods The rabbit BMSC were isolated and were induced into endothelial cells. These cells were divided into four groups. （1） BMSC group cultured under normoxia; （2） BMSC group cultured under hypoxia; （3） BMSC＋EC group co-cuhured under normoxia, （4） BMSC＋EC group co-cuhured under hypoxia. Inverted microscope and MIT method were applied to observe the effects of hypoxia and induced autologous EC on the cellular compatibility of BMSC. Results Endothelial cells could be induced from BMSC. Inverted microscope showed a cellular compatible growing status of BMSC and EC. The ability of proliferation of groups cultured under hypoxia were stronger than groups cultured under normoxia （P〈0.01）, but there was no significant difference between BMSC groups and BMSC＋EC groups （P〉0.05）. Conclusion The hypoxia can increase the proliferation of BMSC. While the endothelial cells induced from BMSC can not.
出处
《组织工程与重建外科杂志》
2010年第6期315-318,共4页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
骨髓基质干细胞
内皮细胞
低氧
联合培养
Bone marrow stromal cell
Endothelial cell
Hypoxia
Co-culture
