摘要
本工作采用酿酒酵母细胞表达载体pESC和植物细胞表达载体pPZP211分析了拟南芥AZI1基因对真菌的抗性功能。半乳糖诱导产生的AZI1蛋白可以使酵母细胞的生长能力明显降低。DAB和台酚蓝染色结果显示用蒜薹灰霉菌孢子处理Col-0野生型植株叶片后被侵染部位只能产生少量H2O2,病原体可以扩散,而AZI1基因过表达植株叶片在侵染部位有大量H2O2产生,着色较深,表明转化体能够以局部细胞的死亡来阻止病原体侵染周围的细胞。在Col-0野生型植株中,AZI1基因的表达受外源水杨酸诱导,24h后达到峰值。以上结果说明AZI1基因在拟南芥对生物胁迫因素的应答过程中具有重要作用。
Resistance ofArabidopsis AZI1 to fungus was analyzed with Saccharomyces cerevisiae expression vector pESC and plant expression vector pPZP211. Synthesis of AZI 1 after induction with galactose resulted in remarkable decrease of the growth ability of yeast cells. DAB and trypan blue staining showed that only small amounts of H2O2 were produced at the infection sites of Botrytis cinerea spores and the pathogens could spread to surrounding cells in leaves of wild-type Col-0 plants. In contrast to this, the infection sites on leaves of AZI1 overexpression Col-0 plants accumulated large amounts of H2O2 and were stained intensively by DAB, indicating the transformants could prevent the spread of pathogen by the death of local cells around the infection sites. In wild-type Col-0 plants, the expression of AZI1 could be induced by salicylic acid and peaked after 24 h. All these results suggested that AZI1 play an important role in defense system of Arabidopsis against biotic stresses.
出处
《植物生理学报》
CAS
CSCD
北大核心
2011年第1期57-62,共6页
Plant Physiology Journal
基金
国家自然科学基金(30870194)
陕西省重点实验室研究计划(08JZ70
2010JS090)
陕西省科学技术研究发展计划(攻关计划2010K16-04-01)
关键词
AZI1基因
过表达
酿酒酵母
蒜薹灰霉菌
水杨酸
H2O2
AZI1
overexpression
Saccharomyces cerevisiae
Botrytis cinerea Pers. ex of garlic sprout
sali-cylic acid
H2O2
