摘要
采用ＦＴ－Ｒａｍ ａｎ 光谱仪对蛋白质样品进行了多次扫描，以去卷积谱、二阶及四阶导数谱为参考确定各子峰峰位。采用酰胺Ⅰ带的原始谱图作曲线拟合， 并以子峰面积表征对应二级结构含量。用所建立的方法研究了多种蛋白质二级结构，
In this work,proteins have been scanned by FT Raman spectrometer.Deconvolved spectra,second derivative or fourth derivative spectra were employed to determine positions of individual components.Curve fitting procedures were carried out to amide Ⅰ band of original spectra.The broad band was resolved into several components.The resolved band components were assigned to specific protein conformations.The areas of individual components are used to estimate the percentage of the relative secondary structure.Results are in reasonable agreement with data obtained by other methods.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
1999年第5期674-676,共3页
Spectroscopy and Spectral Analysis
