摘要
[目的]用HPLC法测定贯叶金丝桃(Hypericum perforalurnL.)提取液中金丝桃素含量,以期为贯叶金丝桃的开发利用提供理论依据。[方法]HPLC条件为:岛津VP-ODS C18(4.6 mm×150 mm,0.5μm)色谱柱;流动相为甲醇-乙酸乙酯-磷酸盐缓冲溶液(V∶V=60∶20∶20,pH=2.0);流速为1.0 ml/min;柱温25℃;检测波长590 nm;进样量10μl。[结果]贯叶金丝桃提取液在金丝桃素含量为2.56～19.20μg/ml浓度范围内与峰面积呈良好的线性关系(r=0.999 9),测得金丝桃素的含量为0.79 mg/g,提取率0.079%。[结论]该方法精密度良好,重现性好,稳定性好,适合贯叶金丝桃中金丝桃素含量的测定。
[Objective]To determine the content of hypericin in Hypericum perforalurn L.by HPLC,so as to provide references for its development and utihzation.[Method]The content of hypericin was determined by HPLC,and HPLC conditions were as follows： column of SHIMADZU VP-ODS C18 column,mobile phase of methanol-ethyl acetate-phosphate buffer（V∶V=60∶20∶20,pH=2.0）,flow rate of 1.0 ml/min,column temperature of 25 ℃,detection wavelength of 590 nm and injecting amount of 10 μl.[Result]In the range of 2.56-19.20 μg/ml,the linear relationship of hypericin was good（r=0.999 9）.The content of hypericin in Hypericum perforalurn L.was 0.79 mg/g and the yield of it was 0.079%.[Conclusion]The method established in this study with good precision,reproducibility and stability is favorable for the determination of hypericin in Hypericum perforalurn L..
出处
《安徽农业科学》
CAS
北大核心
2010年第34期19350-19351,共2页
Journal of Anhui Agricultural Sciences
基金
吉林省教育厅"十一五"规划重点项目(2007238)
