摘要
目的：体外观察瑞香狼毒醇提物AESC,AESC-1,AESC-2的抗肿瘤作用。方法：采用MTT和SRB法观察AESC,AESC-1,AESC-2对5种肿瘤细胞（A549,NCI-H157,NCI-H460 3种肺癌细胞株,BEL-7402,SK-HEP-1 2种肝癌细胞株）的抑制率,计算IC50,GI50,TGI和LC50。结果：AESC,AESC-1,AESC-2对各细胞株均具有抑制作用,并具有一定的时间依赖性。作用72 h,100,200 mg.L-1各受试药对各细胞株（除A549外）的抑制率在64.82%92.27%。AESC和AESC-2对NCI-H460和BEL-7402细胞株的IC50〈20 mg.L-1,AESC-1对SK-HEP-1细胞株IC50〈20 mg.L-1。AESC,AESC-1,AESC-2对敏感细胞株的平均GI50〈40 mg.L-1,平均TGI〈90 mg.L-1,平均LC50〈150 mg.L-1。AESC和AESC-2对敏感细胞株的平均GI50,TGI和LC50值相似。结论：AESC,AESC-1,AESC-2均具有明显抗肿瘤作用,且对不同细胞株敏感性不同;AESC-2与AESC抗肿瘤作用方式相似,AESC的抗肿瘤活性部位可能主要存在于AESC-2。
Objective： To observe the anti-tumor effect of alcohol extract of Stellera chamaejasme-AESC,AESC-1 and AESC-2 in vitro.Method： Inhibition rates of AESC,AESC-1,AESC-2 on five tumor cells（A549,NCI-H157,NCI-H460,BEL-7402 and SK-HEP-1 cell lines） were observed and IC50,GI50,TGI50 and LC50 were calculated with the methods of MTT and SRB.Result： AESC,AESC-1,AESC-2 have inhibitory effect on all of 5 cell lines and have a certain time-dependent nature.On 72 hours,inhibition rates of 100,200 mg·L-1of various extracts on each cell line（except A549） were between 64.82% and 92.27%.IC50 of AESC and AESC-2 on NCI-H460 and BEL-7402 cell lines is less than 20 mg·L-1,IC50 of AESC-1 on SK-HEP-1 cell line is less than 20 mg·L-1.The mean GI50,TGI and LC50 of AESC,AESC-1,AESC-2 on sensitive cell lines were less than 40,90,150 mg·L-1respectively.Conclusion： AESC,AESC-1 and AESC-2 all have significant anti-tumor effect and have the different sensitivity to different cell lines;AESC-2 has the similar anti-tumor manner to the AESC and the anti-tumor active fraction of AESC may be mainly present in the AESC-2.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第22期3048-3051,共4页
China Journal of Chinese Materia Medica
基金
中国博士后基金项目(20090450550)
中国中医科学院基本科研业务自选课题项目(Z02065)
关键词
瑞香狼毒
抗肿瘤
Stellera chamaejasme； anti-tumor；
