摘要
目的观察牛至油对肿瘤细胞株的生长抑制作用。方法采用MTT法检测不同浓度的牛至油对体外培养的多种肿瘤细胞株的生长抑制作用,计算半数抑制浓度(IC50)。结果不同浓度牛至油作用后,人肝癌细胞系HepG2、人子宫颈癌细胞系JTC-26和肺癌细胞系A549出现增殖阻滞。MTT法确定牛至油对肝癌HepG2的IC50为118μg/ml;人子宫颈癌JTC-26的IC50为118μg/ml;肺癌A549的IC50为59μg/ml。结论牛至油具有体外抗肿瘤活性。
Objective To observe the inhibitory effect of Origanum oil on three types of tumor cell lines in vitro.Method MTT assay was used to test the inhibitory effect of Origanum oil on different types of tumor cell lines in vitro with different concentration and IC50 was calculated.Result After Origanum oil treatment,the human hepatoma cell line HepG2,human cervical cancer cell line JTC-26 and lung cancer cell line A549 occurred proliferation blocking.The IC50 of Oregano oil for the liver cancer line HepG2,human cervical cancer cell line JTC-26 and lung cancer cell line A549 were 118 μg/ml,118 μg/ml and 59 μg/ml respectively by MTT method.Conclusion Oregano oil has anti-tumor activity in vitro.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第12期1101-1102,1106,共3页
Chinese Journal of Microecology
