摘要
人红细胞带３ 蛋白胞质段（ｃｙｔｏｐｌａｓｍｉｃｄｏｍａｉｎ ｏｆ ｂａｎｄ ３，ｃｄｂ３） 起着将膜与膜骨架、细胞内环境相联系的重要作用．以带３ 蛋白全长基因为模板， 用ＰＣＲ方法扩增出ｃｄｂ３ 片段， 克隆至ｐＲＳＥＴ表达质粒上， 转化大肠杆菌ＢＬ２１ （ＤＥ３） ． 转化菌经诱导表达出较高含量的ｃｄｂ３ 蛋白， 纯化后，
The cytoplasmic domain of human erythrocyte band 3 (cdb3) plays an important role in connection of the membrane with the membrane skeleton, and environment inside the cell as well. With whole length of band 3 gene, cdb3 gene was amplified by PCR and then was cloned into pRSET plasmid. The plasmid was introduced into E.coli BL21(DE3). The cdb3 expression proteins were purified and its function was verified according to the ability to inhibit aldolase.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第5期492-494,共3页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
带3蛋白
胞质段
质粒
表达
红细胞
band 3 protein, cytoplasmic domain, plasmid, expression
