摘要
通过在湿法合成的二水磷酸氢钙膏体中加入中药骨碎补的提取物,作为磷酸钙骨水泥(Calcium Phosphate Cement,CPC)原料之一,分别制备0、5wt%、10wt%和15wt%的载骨碎补磷酸钙骨水泥.采用Gilmore针、X射线衍射仪、红外光谱仪、万能材料试验机、扫描电子显微镜和紫外分光光度计研究载骨碎补CPC的理化性能和药物释放;体外培养MC-3T3成骨细胞,进行Alamar Blue和碱性磷酸酶检测,研究载骨碎补CPC对成骨细胞增殖和分化的影响,扫描电子显微镜观察细胞形貌.结果表明:随骨碎补浓度的增加,CPC凝结时间明显延长,其抗压强度显著提高;骨碎补促进初期CPC的水化,却阻碍了α-磷酸三钙的转化,且随骨碎补浓度增大作用愈明显,骨碎补不影响CPC水化后的相成分;含骨碎补CPC的微观形貌中出现片状和针状晶体,结构较空白CPC更加致密;药物释放分为突释和缓释两个阶段,符合Higuchi基质扩散释放模型;载骨碎补CPC对成骨细胞的作用呈剂量和时间依赖关系,培养5d时浓度为5wt%和10wt%的CPC较明显地促进细胞增殖,7d时载骨碎补CPC的细胞增殖较稳定,细胞分化能力无显著性差异;成骨细胞在载骨碎补CPC表面生长形态良好,表明该材料具有较好的生物相容性.
The extract of drynaria,a traditional Chinese medical herb,was added into dicalcium phosphate dihydrate （DCPD） with concentration of 5wt%,10wt%and 15wt%to form drug-loaded calcium phosphate cement （CPC）.The effects of drynaria on the physichemical properties and drug release behavior of CPC were characterized by Gilmore needle tests,X-ray diffraction（XRD）,Fourier transform infrared spectroscopy（FTIR）,compressive tests,scanning electron microscope（SEM） and UV-Vis spectrophotometry（UV-Vis）.The effects of drynarialoaded CPC on the morphology,proliferation,and differentiation of MC-3T3 osteoblast were evaluated by SEM, Alamar blue assay,and alkaline phosphatase assay,respectively.It was found that the addition of drynaria increased the setting time and compressive strength of CPC in dose-dependent manners.The addition of drynaria also accelerated the initial hydration of CPC,but inhibited the subsequent conversion ofα-tricalcium phosphate,which were closely related to the concentration of drynaria.In comparison,the phases of CPC hydration products were not affected by drynaria.Drynaria-loaded CPC consisting of platy and acicular crystals were more compact than the control CPC.The drug release profile exhibited an initial fast release stage followed by a slow release stage,which was fit to the diffusion-controlled Higuchi model.The effect of drynaria-loaded CPC on osteoblasts was time- and dose-dependent.CPC loaded with 5wt%and 10wt%drynaria significantly promoted osteoblast proliferation after culture for 5d（P0.01）.After cultured with drynaria-loaded CPC for 7d,osteoblasts proliferated steadily.Osteoblasts cultured on drynaria-loaded CPCs and control CPC showed similar differentiation.Osteoblasts with a normal morphology were observed on the surface of drynaria-loaded CPC,indicating an excellent biocompatibility.
出处
《无机材料学报》
SCIE
EI
CAS
CSCD
北大核心
2011年第1期29-37,共9页
Journal of Inorganic Materials
基金
国家自然科学基金(30470484,50975239)
教育部新世纪人才基金(NcET-05-0797)~~
关键词
磷酸钙骨水泥
骨碎补
理化性能
生物相容性
calcium phosphate cement
drynaria
physichemical properties
biocompatibility
