摘要
目的观察针刺手厥阴经穴在大鼠缺血再灌注损伤过程中对体内钙网蛋白(CRT)含量及蛋白表达的影响及其作用机理。方法 50只Wistar大鼠随机分为假手术组、缺血再灌注模型组、针刺内关治疗组、针刺郄门治疗组和针刺合谷对照组,每组10只。假手术组只穿线不结扎冠状动脉,其余各组制备缺血再灌注动物模型,其中3个针刺穴位组分别于针刺20min后结扎冠状动脉左前降支40min,心电图监测,再次针刺上述穴位20min,松扎,恢复冠脉灌流。假手术组于穿线后100min、模型组和各针刺穴位组于再灌注后60min,腹腔静脉取血并摘取心脏,观察血清CRT含量,检测心肌组织CRT蛋白表达的变化。结果缺血再灌注模型组心肌组织CRT表达与假手术组比较有统计学差异(P<0.05);针刺内关治疗组、针刺郄门治疗组血清CRT含量明显升高,心肌组织CRT表达呈高表达状态,与缺血再灌注模型组比较均有统计学差异(P<0.01);针刺合谷对照组与缺血再灌注模型组近似,两者比较无统计学差异(P>0.05)。结论针刺手厥阴经穴可明显提高体内CRT含量,促进CRT表达的上调,减轻了缺血再灌注造成心肌损伤的程度。
Objective To study the effect of electroacupuncture （EA） at points of the Pericardium Meridian of Hand-Jueyin on the calreticulin （CRT） content and expression in myocardial ischemia reperfusion injury rats and explore its mechanism. Methods Fifty Wistar rats were randomized into sham-operated group,ischemia reperfusion model group,EA Neiguan （PC6） group,EA Ximen （PC5） group,and EA Hegu （LI4） group,10 in each. The sham-operated group was operated only with threading but without ligation of coronary artery,and the other groups were all made ischemia reperfusion models. The left descending anterior branch of the coronary artery of the three acupuncture groups were ligated twenty minutes after acupuncture for 40 minutes. Under the observation of electrocardiogram,the above-mentioned points were treated with acupuncture again for twenty minutes,and then the ligation was stopped for reperfusion. One hundred minutes after threading in the sham-operated group and 60 minutes after reperfusion in the model and all acupuncture groups,the abdominal venous blood and myocardial sample were taken for observing CRT content and expression. Results In the ischemia reperfusion model group,there was significant difference in the myocardial CRT expression,compared with the sham-operated group （P〈0.05）. In the EA Neiguan （PC6） and EA Ximen （PC5） groups,the serum CRT content was significantly increased and myocardial CRT expression was strengthened,compared with the ischemia reperfusion model group （P〈0.01）. In the EA Hegu （LI4） group,the result was similar with that of the ischemia reperfusion model group （P〉0.05）,without statistical difference. Conclusion EA stimulation of points of the Pericardium Meridian can significantly increase the content and expression of CRT,making the myocardial injury caused by ischemia reperfusion reduced.
出处
《中医杂志》
CSCD
北大核心
2011年第3期227-230,共4页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30873240)
山西省自然科学基金资助项目(2010011056-1)
关键词
针刺
心肌缺血
再灌注损伤
钙网蛋白
大鼠
内关穴
郄门穴
electroacupuncture
myocardial ischemia reperfusion injury
calreticulin
rats
Neiguan （PC6）
Ximen （PC5）
