摘要
目的:通过检测非甾体类抗炎药NS398对前列腺癌动物模型中RECK基因表达的调控,探讨其可能的抗肿瘤机制。方法:成瘤裸鼠分为实验组和对照组,实验组裸鼠予口咽管下喂服NS398,每天剂量0.1 mg/g体重,分别喂服10 d、20 d、30 d,对照组不予喂药,各组均在30 d后处死裸鼠;抽提各组肿瘤组织总RNA,RT-PCR检测RECK基因与MMP-9的表达;抽提各组肿瘤组织总蛋白,应用W estern印迹法检测RECK蛋白的表达。结果:实验组肿瘤组织中RECK基因的表达量较对照组明显升高,而MMP-9的表达量明显降低,且其变化与用药时间有明显的相关性。结论:NS398对前列腺癌的发生及转移有明显的抑制作用,其具体的作用机制可能与其诱导RECK基因的表达,从而抑制MMP-9的表达有关。
Objective：To investigate the regulatory effect of the nonsteroidal anti-inflammatory drug NS398 on the expression of the RECK gene in the animal model of prostate cancer.Methods：Nude mouse models of prostate cancer were divided into an experimental and a control group,the former fed with NS398 at 0.1 mg/g per day for 10,20 and 30 days,while latter left without medication.All the mice were killed at 30 days,the mRNA expressions of RECK and MMP-9 in the tumor tissues measured by RT-PCR,and the protein level of RECK evaluated by Western blot.Results：Both the mRNA and protein expressions of RECK were increased,while the level of MMP-9 decreased,in an obviously time-dependent manner in the experimental group as compared with the control.Conclusion：NS398 obviously inhibits the pathogenesis and metastasis of prostate cancer,which may be attributed to its induction of the expression of the RECK gene and suppression of the expression of MMP-9.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2010年第12期1079-1082,共4页
National Journal of Andrology
基金
国家杰出青年资金(30225046)~~
